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GR37

Sigma-Aldrich

Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)

liquid, clone 9A7γE10.4, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

9A7γE10.4, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

chicken, hamster, mouse, porcine, rat, amphibian, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... VDR(7421)

General description

Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.
Recognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.
This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).

Immunogen

purified, chick intestine vitamin D receptor

Application

Frozen Sections (1:50-1:100)

Gel Shift (see comments)

Immunoblotting (1:200-1:400)

Immunoprecipitation (10 µl/mg protein lysate)

Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Breast carcinoma tissue (see comments)

Other Notes

Brown, A.J. 1999. Nephrol. Dial. Transplant.14, 11.
Fleet, J.C. 1999. Nutr. Rev.57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res.47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.
Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jae Young So et al.
The Journal of steroid biochemistry and molecular biology, 148, 111-121 (2014-12-30)
Tumor-initiating cells (also known as cancer stem cells) are the subpopulation of cells shown to be responsible for tumor initiation, maintenance and recurrence. In breast cancer, CD44(+)/CD24(-/low) cells were identified as tumor-initiating cells. We previously reported that a Gemini vitamin
José L Costa et al.
BMC genomics, 10, 499-499 (2009-10-30)
The active form of Vitamin D, 1,25-dihydroxyvitamin D3 (1,25D), has strong anti-proliferative effects, yet the molecular mechanisms underneath this effect remain unclear. In contrast, the molecular mechanism of 1,25D for the regulation of calcium homeostasis has principally been resolved, demonstrating

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