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MILLIPLEX® Human Complement Panel 1 - Immunology Multiplex Assay

The Human Complement Panel 1 Bead-Based Multiplex Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of complement proteins and factors in human serum, plasma and cell culture samples.

Synonym(s):

Human complement multiplex kit, Luminex® human complement immunoassay, Millipore human complement panel

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 96%
(Mannose-Binding Lectin (MBL))
sensitivity: 0.0051 ng/mL
(Complement C5a)
sensitivity: 0.026 ng/mL
(Adipsin (Complement Factor D))
sensitivity: 0.040 ng/mL
(Mannose-Binding Lectin (MBL))
sensitivity: 0.22 ng/mL
(Complement Factor I)
sensitivity: 0.31 ng/mL
(Complement C2)
sensitivity: 0.36 ng/mL
(Complement C4b)
sensitivity: 1.040 ng/mL
(Complement C5)
standard curve range: 0.069-50 ng/mL
(Adipson/ Complement Factor D)
standard curve range: 0.07-50 ng/mL
(Complement C2)
standard curve range: 0.137-100 ng/mL
(Mannose-Binding Lectin (MBL))
standard curve range: 0.69-500 ng/mL
(Complement Factor I)
standard curve range: 1.37-1,000 ng/mL
(Complement C4b)
standard curve range: 2.74-2,000 ng/mL
(Complement C5)
standard curve range: 4.12-30,000 pg/mL
(Complement C5a)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

2-8°C

Related Categories

General description

The complement system consists of many plasma proteins that assist the ability of phagocytic cells and antibodies to clear pathogens. There are three different simultaneous pathways. The classical pathway is stimulated by antigen-antibody complexes; the alternative pathway spontaneously activates on contact with pathogenic cell surfaces; and the mannose-binding lectin (MBL) pathway recognizes mannose sugars usually present only on pathogenic cell surfaces. Because of the potential to be extremely damaging to host tissues, complement system activation must be tightly controlled. The regulating complement control proteins, including CD59, also known as protectin, are present at a higher concentration in the blood plasma than the complement proteins themselves. The complement system is thought to play a key role in many diseases with an immune component, such as asthma and many autoimmune diseases, including SLE, IBD and multiple sclerosis. It is also becoming increasingly associated with neurological disease, such as Alzheimer′s disease and conditions such as spinal cord injuries.

MILLIPLEX® Complement Panel 1 Bead Panel is an 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: C2, C4B, C5, C5a, Adipsin/Complement Factor D, MBL and Complement Factor I.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Specificity

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: C2, C4b, C5, C5a, Adipsin/Factor D, Mannose-Binding Lectin (MBL), and Factor I
  • Recommended Sample type: Serum, plasma, and tissue/cell culture supernatant
  • Recommended Sample dilution: 1:200 diluted plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
  • Assay Run Time: Overnight or two-hour primary incubation
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Inflammation
  • Note: To avoid complement activation in samples, which will affect the results, samples should be thawed as rapidly as possible, put on ice and assayed immediately

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

Certificates of Analysis (COA)

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Frank Sebastian Fröschen et al.
Diagnostics (Basel, Switzerland), 11(3) (2021-04-04)
The role and diagnostic value of the synovial complement system in patients with low-grade periprosthetic joint infection (PJI) are unclear. We sought to evaluate, for the first time, the usefulness of synovial complement factors in these patients by measuring the
Ngo Truong Giang et al.
Scientific reports, 10(1), 14923-14923 (2020-09-12)
The complement system may be crucial during dengue virus infection and progression to severe dengue. This study investigates the role of MBL2 genetic variants and levels of MBL in serum and complement proteins in Vietnamese dengue patients. MBL2 genotypes (- 550L/H
Pavan Bhargava et al.
Multiple sclerosis (Houndmills, Basingstoke, England), 27(4), 509-518 (2020-07-17)
Synaptic loss is a feature of multiple sclerosis pathology that can be seen even in normal-appearing gray matter. Opsonization of synapses with complement components may underlie pathologic synapse loss. We sought to determine whether circulating neuronal-enriched and astrocytic-enriched extracellular vesicles
Manjunath Ramanjaneya et al.
Frontiers in endocrinology, 13, 918320-918320 (2022-08-02)
Complement factors mediate the recruitment and activation of immune cells and are associated with metabolic changes during pregnancy. The aim of this study was to determine whether complement factors in the maternal serum and follicular fluid (FF) are associated with
Laura McCulloch et al.
F1000Research, 8, 1039-1039 (2019-11-12)
Background: Blockade of the cytokine interleukin-1 (IL-1) with IL-1 receptor antagonist (IL-1Ra) is a candidate treatment for stroke entering phase II/III trials, which acts by inhibiting harmful inflammatory responses.  Infection is a common complication after stroke that significantly worsens outcome
View All Related Papers

Related Content

Immunology Multiplex Assays | MILLIPLEX® Assays

Immunology multiplex assays, like MILLIPLEX® assays, allow researchers to investigate more immune biomarkers simultaneously, saving time and resources. This is especially critical to immunology research, where numerous analytes play a role in different pathways.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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