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HPTP1MAG-66K

Millipore

MILLIPLEX® Human Pituitary Magnetic Bead Panel 1 - Endocrine Multiplex Assay

The Human Pituitary Panel 1, using the Luminex xMAP technology, enables the simultaneous analysis of 7 protein biomarkers in human serum, plasma and cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 105%
(TSH)
accuracy: 77%
(AGRP)
accuracy: 87%
(LH)
accuracy: 97%
(FSH)
sensitivity: 0.02 μIU/mL
(minDC+2SD; TSH)
sensitivity: 0.02 mIU/mL
(minDC+2SD; FSH)
sensitivity: 0.02 mIU/mL
(minDC+2SD; LH)
sensitivity: 1.12 pg/mL
(minDC+2SD; AGRP)
sensitivity: 2.4 pg/mL
(minDC+2SD; ACTH)
sensitivity: 6.88 pg/mL
(minDC+2SD; GH)
sensitivity: 84.42 pg/mL
(minDC+2SD; CNTF)
standard curve range: 0.024-100 mIU/mL
(FSH)
standard curve range: 0.039-160 μIU/mL
(TSH)
standard curve range: 0.049-200 mIU/mL
(LH)
standard curve range: 122-500,000 pg/mL
(CNTF)
standard curve range: 2.4-10,000 pg/mL
(AGRP)
standard curve range: 2.4-10,000 pg/mL
(GH)
standard curve range: 3.0-12,500 pg/mL
(ACTH)
inter-assay cv: <15%
intra-assay cv: <10%
(ACTH)
inter-assay cv: <15%
intra-assay cv: <10%
(AgRP)
inter-assay cv: <15%
intra-assay cv: <10%
(CNTF)
inter-assay cv: <15%
intra-assay cv: <10%
(FSH)
inter-assay cv: <15%
intra-assay cv: <10%
(GH)
inter-assay cv: <15%
intra-assay cv: <10%
(LH)
inter-assay cv: <15%
intra-assay cv: <10%
(TSH)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Called the “master gland” because it controls many other endocrine glands, the pituitary secretes several key hormones that play important roles in the regulation of metabolism, growth, and reproduction. Piuitary hormones are responsible for the stimulation of the adrenal gland (ACTH), thyroid gland (TSH), ovaries and testes (FSH, LH), as well as blood pressure and the amount of water excreted by the kidneys. Through its connection with the pituitary, the hypothalamus modulates endocrine function by detecting hormone levels.

The MILLIPLEX® Human Pituitary Panel 1 is a 7-plex kit to be used for the simultaneous measurement of any or all of the following analytes in human serum, plasma or cell/tissue culture samples: ACTH, AGRP, CNTF, FSH, GH, LH, and TSH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Endocrine

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: ACTH, Agouti-Related Protein (AGRP), CNTF, FSH, GH, LH, TSH
  • Recommended Sample Type: Human serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysates and supernatants Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media
  • Assay Run Time: Overnight (16-18 hr) at 2-8°C
  • Research Category: Endocrine
  • Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate
Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

Certificates of Analysis (COA)

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Contact Technical Service