MAB1096
Anti-Tumor Necrosis Factor-α Antibody, clone 195
clone 195, Chemicon®, from mouse
Synonym(s):
TNF-alpha
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
195, monoclonal
species reactivity
human
should not react with
pig, monkey, canine, mouse, rat, baboon
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable
isotype
IgG3
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TNF(7124)
Related Categories
Specificity
The antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon.
SPECIES REACTIVITIES:
Not mouse, rat, canine, porcine, baboon or rhesus-monkey.
SPECIES REACTIVITIES:
Not mouse, rat, canine, porcine, baboon or rhesus-monkey.
Immunogen
Human, recombinant tumor necrosis factor-alpha produced in E. coli.
Application
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
This Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.
Western Blot
Immunocytochemistry
Immunohistochemistry
Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunohistochemistry
Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].
Optimal working dilutions must be determined by end user.
Linkage
Replaces: 04-1114
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.
Storage and Stability
Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Acute bronchial obstruction in sheep: histopathology and gland cytokine expression.
Robert A Cox, Ann S Burke, Gloria Oliveras, Perenlei Enkhbaatar, Lillian D Traber et al.
Experimental Lung Research null
Monocyte-mediated drug-dependent cellular cytotoxicity: effects on different WEHI 164 target cell lines.
Austgulen, R, et al.
Cancer Immunology, Immunotherapy, 22, 176-180 (1986)
Human lymphotoxin.
Aggarwal, B B
Test, 116, 441-448 (1985)
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