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MAB1330

Sigma-Aldrich

Anti-Collagen Type II Antibody, clone COLL-II

ascites fluid, clone COLL-II, Chemicon®

Synonym(s):

Anti-ANFH, Anti-AOM, Anti-COL11A3, Anti-SEDC, Anti-STL1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

COLL-II, monoclonal

species reactivity

sheep, human, bovine, rat, pig, mouse, canine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

isotype

IgG1

NCBI accession no.

NM_001844.4
NM_033150.2

UniProt accession no.

P02458

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL2A1(1280)

Specificity

Reacts with both pepsin-solubilized and CNBr-cleaved human and bovine collagen type II. No cross-reactivity with collagen types I, III, V and IX.

Immunogen

Human (cartilage specific) CNBr-cleaved collagen II.

Application

Immunofluorescence: 1:2-1:5 with fresh, frozen or acetone-fixed material. Mild pepsin digestion is recommended to enhance staining.

Western Blot: 1:1000-1:2000 with chemiluminescent detection

ELISA at 1:100-1:500, pepsin treated samples.





Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Collagen Type II Antibody, clone COLL-II is validated for use in ELISA, IH for the detection of Collagen Type II.

Physical form

Ascites fluid containing no preservatives.
Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Cartilage in lung or fetus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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ALK1 opposes ALK5/Smad3 signaling and expression of extracellular matrix components in human chondrocytes.
Kenneth W Finnson,Wendy L Parker,Peter ten Dijke,Midory Thorikay,Anie Philip
Journal of Bone and Mineral Research null
Alpha-smooth muscle actin expression and structure integrity in chondrogenesis of human mesenchymal stem cells
Hung, Shih-Chieh, et al
Cell and Tissue Research, 324, 457-466 (2006)
Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads.
Hsiao-Li Ma, Shih-Chieh Hung, Shan-Yang Lin, Yuh-Lien Chen, Wai-Hee Lo
Journal of Biomedical Materials Research Part A null
Preservation of the chondrocyte's pericellular matrix improves cell-induced cartilage formation.
Lucienne A Vonk,Behrouz Zandieh Doulabi,Chunling Huang,Marco N Helder,Vincent Everts,Ruud A Bank
Journal of Cellular Biochemistry null
Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
Miller, E.J.
Biochemistry, 11, 4903-4909 (1972)
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