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MAB1548

Sigma-Aldrich

Anti-Myosin Antibody, heavy chain β

culture supernatant, clone 5B9 (2C8), Chemicon®

Synonym(s):

Anti-CMD1S, Anti-MPD1, Anti-MYHCB, Anti-SPMD, Anti-SPMM

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

5B9 (2C8), monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_000257.2

UniProt accession no.

P12883

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

rat ... Myh7(29557)

Specificity

Recognizes the S1/S2 junction of human ventricular myosin heavy chain beta

Immunogen

Epitope: heavy chain beta
Human ventricular myosin

Application

Detect Myosin using this Anti-Myosin Antibody, heavy chain β validated for use in WB, IH.
Immunohistochemistry: Neat (undiluted)
Western blot: 1:10

Optimal dilutions must be determined by the end user.

Physical form

Supernatant in RPMI 1640, 10% FCS, 0.01% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Imaging of myocardial infarction in dogs and humans using monoclonal antibodies specific for human myosin heavy chains.
Leger, Joc., et al.
Journal of the American College of Cardiology, 18, 473-484 (1991)
Fiber types and myosin types in human atrial and ventricular myocardium. An anatomical description.
Bouvagnet, P, et al.
Circulation Research, 55, 794-804 (1984)
Histochemistry of ventricular heavy-chain myosins in cardiomyopathic Syrian hamsters treated with D-600.
Jasmin, G, et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 188, 142-148 (1988)
C A Dechesne et al.
Circulation research, 57(5), 767-775 (1985-11-01)
Rat, rabbit, pig, and bovine atrial myocardia were investigated with anti-alpha and anti-beta myosin heavy chain monoclonal antibodies. Analysis of atrial fibers by indirect immunofluorescence and assay of myosin heavy chains in tissue micro samples by immunoaffinity chromatography revealed both
Siva K Panguluri et al.
American journal of physiology. Heart and circulatory physiology, 304(12), H1651-H1661 (2013-04-16)
Ventricular arrhythmias account for high mortality in cardiopulmonary patients in intensive care units. Cardiovascular alterations and molecular-level changes in response to the commonly used oxygen treatment remains unknown. In the present study we investigated cardiac hypertrophy and cardiac complications in
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