MAB1683
Anti-Ankyrin Antibody, clone Ank016
clone Ank016, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Ank016, monoclonal
species reactivity
rat, human, rabbit, pig, mouse, bovine, canine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ANK1(286)
Related Categories
Specificity
Reacts with the intracellular ankyrin (216 kDa). Ankyrin consists of a single peptide of M.W. 215 kDa known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues).
Immunogen
Purified human erythroid cells ankyrin
Application
Anti-Ankyrin Antibody, clone Ank016 is an antibody against Ankyrin for use in ELISA, FC, IP, WB & IC.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Western blotting at 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals.
ELISA: 1:1000
Immunoprecipitation: 1:100
Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10′ RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular.
Flow cytometry: 1:500
Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended.
RIA: 1:5000
Optimal working dilutions must be determined by end user.
ELISA: 1:1000
Immunoprecipitation: 1:100
Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10′ RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular.
Flow cytometry: 1:500
Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended.
RIA: 1:5000
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Purified immunoglobulin from culture supernatant. Liquid in 10 mM sodium phosphate, pH 7.5 and 150mM NaCl with 0.01% NaN3.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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P A Lang et al.
Cell death and differentiation, 12(5), 415-428 (2005-03-05)
Hyperosmotic shock, energy depletion, or removal of extracellular Cl(-) activates Ca(2+)-permeable cation channels in erythrocyte membranes. Subsequent Ca(2+) entry induces erythrocyte shrinkage and exposure of phosphatidylserine (PS) at the erythrocyte surface. PS-exposing cells are engulfed by macrophages. The present study
Spectrin-based membrane skeleton: a multipotential adaptor between plasma membrane and cytoplasm.
Bennett, V
Physiological Reviews, 70, 1029-1065 (1990)
The lymphoma transmembrane glycoprotein GP85 (CD44) is a novel guanine nucleotide-binding protein which regulates GP85 (CD44)-ankyrin interaction.
Lokeshwar, V B and Bourguignon, L Y
The Journal of Biological Chemistry, 267, 22073-22078 (1992)
Capping and the cytoskeleton.
Bourguignon, L Y and Bourguignon, G J
International Review of Cytology, 87, 195-224 (1984)
L Y Bourguignon et al.
Journal of immunology (Baltimore, Md. : 1950), 151(12), 6634-6644 (1993-12-15)
The purposes of this study are to characterize the binding of hyaluronic acid (HA) to mouse T lymphoma cells, to measure changes in intracellular Ca2+ after HA binding, to elucidate the interaction between the HA receptor, GP85(CD44), and ankyrin in
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