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MAB1945

Sigma-Aldrich

Anti-Vitronectin Antibody, clone BV2

clone BV2, Chemicon®, from mouse

Synonym(s):

Serum spreading factor, S-protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BV2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000638.3

UniProt accession no.

P04004

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VTN(7448)

Related Categories

Specificity

Human vitronectin. Specificity determined by ELISA and immunoprecipitation of I125 labeled vitronectin purified from plasma (Zanetti et al., 1994). The antibody reacts with vitronectin both in its native and denatured form.

Immunogen

Purified human plasma vitronectin (Preissner, 1985).

Application

Anti-Vitronectin Antibody, clone BV2 is an antibody against Vitronectin for use in ELISA, FC, IP, WB, IH.
ELISA

Immunoprecipitation

Western blot

Immunohistochemistry/immunofluorescence

Inhibits endothelial cell adhesion and spreading on vitronectin-coated surfaces.

Flow Cytometry

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Format: Purified
Purified immunoglobulin by Protein A chromatography. Liquid in PBS containing 0.05% sodium azide and 0.1% BSA.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Complement activation and inflammatory processes in Drusen formation and age related macular degeneration.
L V Johnson, W P Leitner, M K Staples, D H Anderson
Experimental Eye Research null
R J Falk et al.
The American journal of pathology, 127(1), 182-190 (1987-04-01)
The S protein (S) binds to the attack complex of complement (C5b-9) in plasma preventing cytolysis. Using immunofluorescence microscopy, the authors determined the distribution of S in human renal tissue and its relationship to C5b-9, immunoglobulins, C3, albumin, and fibronectin.
Cell attachment on replicas of SDS polyacrylamide gels reveals two adhesive plasma proteins
Hayman, E.G. et al.
The Journal of cell biology, 95, 20-23 (1982)
Immunohistochemical localization of S protein/vitronectin in human atherosclerotic versus arteriosclerotic arteries
Guettier, C. et al.
Virchows Archiv. A, Pathological Anatomy and Histology, 414, 309-313 (1989)
Physicochemical characterization of human S-protein and its function in the blood coagulation system
Preissner, K.T. et al.
The Biochemical Journal, 231, 349-352 (1985)
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