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MAB2000

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone HB1.1

clone HB1.1, Chemicon®, from mouse

Synonym(s):

CD29

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HB1.1, monoclonal

species reactivity

human, pig

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

suitability

not suitable for activity/function inhibition

NCBI accession no.

NM_002211.2
NM_033666.1
NM_033667.1
NM_033668.1
NM_033669.1
NM_133376.1

UniProt accession no.

P05556

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGB1(3688)

Related Categories

Specificity

Reacts with beta-1 subunit of VLA integrins

Application

Anti-Integrin β1 Antibody, clone HB1.1 detects level of Integrin β1 & has been published & validated for use in FC, IP, WB, IH.
Immunoprecipitation of VLA integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB2000.

Flow cytometry: 10 μg/mL

Western blot

Immunohistochemistry in frozen tissue samples: 5 μg/mL

Induction of cell aggregation: 10 μg/mL. MAB2000 is a potent stimulator of cell aggregation tested in U937, Jurkat and human peripheral blood lymphocytes.

MAB2000 does not affect VLA integrin-mediated cell adhesion to matrix proteins.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Linkage

Replaces: 04-1109

Physical form

Format: Purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hypersialylation of beta1 integrins, observed in colon adenocarcinoma, may contribute to cancer progression by up-regulating cell motility.
Seales, EC; Jurado, GA; Brunson, BA; Wakefield, JK; Frost, AR; Bellis, SL
Cancer Research null
PtdIns(3,4,5)P3 regulates spindle orientation in adherent cells.
Toyoshima, F; Matsumura, S; Morimoto, H; Mitsushima, M; Nishida, E
Developmental Cell null
Hyposialylation of integrins stimulates the activity of myeloid fibronectin receptors.
Semel, AC; Seales, EC; Singhal, A; Eklund, EA; Colley, KJ; Bellis, SL
The Journal of Biological Chemistry null
Pig chondrocyte xenoimplants for human chondral defect repair: an in vitro model.
Fuentes-Boquete, Isaac, et al.
Wound Repair and Regeneration, 12, 444-452 (2004)
Mechanical control of cAMP signaling through integrins is mediated by the heterotrimeric Galphas protein.
Francis J Alenghat,Jessica D Tytell,Charles K Thodeti,Alexandrine Derrien,Donald E Ingber
Journal of Cellular Biochemistry null
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