MAB3140
Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9
clone 4E9, Chemicon®, from mouse
Synonym(s):
NHE1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4E9, monoclonal
species reactivity
amphibian, fish, avian, vertebrates (including including fish, amphibians, birds and mammals)
species reactivity (predicted by homology)
mouse, mammals, rat
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
isotype
IgG1
suitability
not suitable for immunoprecipitation
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SLC9A1(6548)
General description
Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.
Specificity
Na+/H+ exchanger, isoform NHE1
Immunogen
Epitope: C-terminus
MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.
Application
Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Works poorly for immunohistochemistry and immunocytochemistry.
Not recommended for immunoprecipitation.
Immunoblotting:
1:1,000 dilution used on a previous lot.
Optimal working dilutions must be determined by the user.
Not recommended for immunoprecipitation.
Immunoblotting:
1:1,000 dilution used on a previous lot.
Optimal working dilutions must be determined by the user.
Quality
Routinely evaluated by Western Blot on Human Kidney lysates.
Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.
Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.
Target description
~100-110 kDa
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Kidney tissue (basolateral membrane)
Kidney tissue (basolateral membrane)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Silencing of sodium/hydrogen exchanger in the heart by direct injection of naked siRNA.
Morgan, PE; Correa, MV; Ennis, IL; Ennis, IE; Diez, AA; Perez, NG; Cingolani, HE
Journal of Applied Physiology (1985)
The spatial organization of proton and lactate transport in a rat brain tumor.
Grillon, E; Farion, R; Fablet, K; De Waard, M; Tse, CM; Donowitz, M; Remy, C; Coles, JA
Testing null
Na(V)1.5 enhances breast cancer cell invasiveness by increasing NHE1-dependent H(+) efflux in caveolae.
Brisson, L, et al.
Oncogene (2010)
A novel chimeric natriuretic peptide reduces cardiomyocyte hypertrophy through the NHE-1-calcineurin pathway.
Kilic, Ana, et al.
Cardiovascular Research, 88, 434-442 (2010)
Stimulation of renal sulfate secretion by metabolic acidosis requires Na+/H+ exchange induction and carbonic anhydrase.
Pelis, RM; Edwards, SL; Kunigelis, SC; Claiborne, JB; Renfro, JL
American Journal of Physiology: Renal Physiology null
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