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MAB345A4

Sigma-Aldrich

Anti-O4 Antibody, clone 81, Alexa Fluor™488 Conjugate

clone 81 (mAB 04), from mouse, ALEXA FLUOR™ 488

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

81 (mAB 04), monoclonal

species reactivity

rat

species reactivity (predicted by homology)

human (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

General description

Oligodendrocytes and astrocytes are derived from common precursor cells, glioblasts (Sommer, 1981). O-Antigens are sulfatides, which function as differentiation markers on the surface of oligodendrocytes of the central nervous system. O4 is formed postnatally (Schachner, 1981) and is a marker for cell bodies and processes of oligodendrocytes types I and II (hairy eyeball type). During oligodendrocyte differentiation, O4 occurs in pro-oligodendrocytes, but not in O-2A-progenitor cells. O4 occurs from day 3 onwards in cell cultures of embryonic mouse brain. Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments.

Specificity

This antibody recognizes Oligodendrocyte marker O4. Also reacts with certain galactolipids in sperm.

Immunogen

Homogenate of white matter of corpus callosum from bovine brain.

Application

Anti-O4 Antibody, clone 81, Alexa Fluor™488 Conjugate detects level of O4 & has been published & validated for use in ICC.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Quality

Evaluated by Immunocytochemistry in undifferentiated rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected O4 in undifferentiated rat oligodendrocyte precursor cells.

Physical form

Precipitation followed by ion exchange purification
Purified mouse monoclonal IgM conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Undifferentiated rat oligodendrocyte precursor cells

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jo Anne Stratton et al.
Cell reports, 24(10), 2561-2572 (2018-09-06)
Pro-regenerative macrophages are well known for their role in promoting tissue repair; however, their specific roles in promoting regeneration of the injured nerve are not well defined. Specifically, how macrophages interact with Schwann cells following injury during remyelination has been

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Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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