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MAB352

Sigma-Aldrich

Anti-Serotonin Antibody, clone YC5/45

culture supernatant, clone YC5/45, Chemicon®

Synonym(s):

5-HT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

YC5/45, monoclonal

species reactivity

mouse, human, rat, lizard, rabbit, pig

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... HTR1A(3350)

Specificity

Recognizes the formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in hemagglutination tests. Shows no reactivity with catecholamine-containing neurons in formaldehyde fixed preparations. MAB352 reacts with serotonin in neurons and recognizes serotonergic sites in fixed tissue sections. By cell agglutination, no cross reactivity with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin.

Immunogen

Serotonin conjugated to BSA.

Application

Anti-Serotonin Antibody, clone YC5/45 is an antibody against Serotonin for use in IH.
Immunohistochemistry at 1:100-1:200 for formaldehyde fixed tissue.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Physical form

Lyophilized Supernatant, preservative free.
Unpurified

Storage and Stability

Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100μL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Reciprocal connections between subdivisions of the dorsal raphe and the nuclear core of the locus coeruleus in the rat.
Myung-A Kim, Hyun S Lee, Beob Y Lee, Barry D Waterhouse
Brain Research null
M Costa et al.
Neuroscience, 7(2), 351-363 (1982-02-01)
Immunoreactive nerve cell bodies and fibres in the intestine have been examined using three antibody preparations raised against 5-hydroxytryptamine. Cross reactivity studies indicate that the substance localized was an hydroxylated indoleamine. In the guinea-pig small intestine, nerve cell bodies were
Retrograde study of projections from the tuberomammillary nucleus to the dorsal raphe and the locus coeruleus in the rat.
Hyun S Lee, Beob Y Lee, Barry D Waterhouse
Brain Research null
Organisation and chemical neuroanatomy of the African elephant (Loxodonta africana) olfactory bulb.
Ngwenya A, Patzke N, Ihunwo AO, Manger PR.
Brain Structure &Amp; Function null
Analysis of gene function in the zebrafish retina.
Jarema Malicki, Hakryul Jo, Xiangyun Wei, Monica Hsiung, Zac Pujic
Methods null

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