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MAB3785

Sigma-Aldrich

Anti-APC Antibody, NT, clone Ali 12.28

clone Ali 12.28, Chemicon®, from mouse

Synonym(s):

Adenomatous Polyposis Coli Protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Ali 12.28, monoclonal

species reactivity

human

should not react with

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000038.3

UniProt accession no.

P25054

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... APC(324)
mouse ... Apc(11789)

General description

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.

Specificity

Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.

Immunogen

Epitope: N-terminus
N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.
Western blot: 4 μg/mL

Immunohistochemistry (Acetone Fixed, Frozen)

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Linkage

Replaces: AB1268

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Analysis Note

Control
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jie Zhong et al.
Nature communications, 13(1), 4961-4961 (2022-08-25)
The adenomatous polyposis coli (APC)-Rho guanine nucleotide exchange factor 4 (Asef) protein-protein interaction (PPI) is essential for colorectal cancer metastasis, making it a promising drug target. Herein, we obtain a sensitivity-enhanced tracer (tracer 7) with a high binding affinity (Kd = 0.078 μM)

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