Anti-GluR2 Antibody, clone 6C4, Alexa Fluor^™ 488 Conjugate

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits.

Epitope: Extracellular domain

Recombinant fusion protein TrpE-GluR2

Immunocytochemistry analysis: A 1:100 dilution from a representative lot detected GluR2 in rat E18 primary cortex cells.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti-GluR2 Antibody, clone 6C4, Alexa Fluor 488 Conjugate is validated for use in IH, IC for the detection of GluR2.

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected GluR2 in adult mouse brain tissue.

The unconjugated parent antibody (Cat. No. MAB397) has an observed molecular weight at ~102 kDa

Protein A purified

Purified mouse monoclonal IgG2a conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Adult mouse brain tissue

ALEXA FLUOR is a trademark of Life Technologies

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.