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MAB4356

Sigma-Aldrich

Anti-DPPA-2 Antibody, clone 6C1.2

clone 6C1.2, from mouse

Synonym(s):

Pluripotent embryonic stem cell-related gene 1 protein, developmental pluripotency associated 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

6C1.2, monoclonal

species reactivity

mouse

technique(s)

western blot: suitable

input

sample type: mouse embryonic stem cell(s)

isotype

IgG2aκ

UniProt accession no.

Q7Z7J5
Q9CWH0

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Dppa2(73703)

General description

DPPA-2 (developmental pluripotency associated-2) is a novel Oct-4-related protein that is localized to the nucleus of mouse fetal germ cells. DPPA-2 has binding sites for both POU and Sox-2 protein domains and is expressed primarily in the developing germ line following gastrulation. These associations suggest that this protein participates in a very strong supportive role in maintaining cell pluripotentiality. In addition to its role as a pluripotency marker, more recent research using cancer cells suggests that DPPA-2 may also act as an embryo-cancer antigen—pointing to the presence of abnormal cell growth and the formation of cancerous cells.

Specificity

Reactivity with other species has not been tested.
This monoclonal antibody recognizes the mouse DPPA-2 protein.

Immunogen

Full length recombinant mouse DPPA-2 protein.

Application

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-DPPA-2 Antibody, clone 6C1.2 is validated for use in WB for the detection of DPPA-2.

Quality

(Based upon representative data from a previous lot).
Western Blotting: Recommended working concentration is 2 µg/mL.

Target description

~34 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and
150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mélanie Eckersley-Maslin et al.
Genes & development, 33(3-4), 194-208 (2019-01-30)
The molecular regulation of zygotic genome activation (ZGA) in mammals remains an exciting area of research. Primed mouse embryonic stem cells contain a rare subset of "2C-like" cells that are epigenetically and transcriptionally similar to the two-cell embryo and thus
Oana Kubinyecz et al.
Development (Cambridge, England), 148(24) (2021-12-22)
Zygotic genome activation (ZGA) represents the initiation of transcription following fertilisation. Despite its importance, we know little of the molecular events that initiate mammalian ZGA in vivo. Recent in vitro studies in mouse embryonic stem cells have revealed developmental pluripotency

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