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MAB5260Z

Sigma-Aldrich

Anti-Nerve Growth Factor Antibody, clone 27/21, azide free

clone 27/21, Chemicon®, from mouse

Synonym(s):

NGF, MAB5260

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

27/21, monoclonal

species reactivity

human, rat, bovine, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
neutralization: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NTF3(4908)

Related Categories

Specificity

Specifically reacts with the beta -subunit of NGF from mouse, in the 2.5S- as well as in the 7S-form.

Immunogen

2.5 S form of mouse NGF.

Application

Detect Nerve Growth Factor using this Anti-Nerve Growth Factor Antibody, clone 27/21, azide free validated for use in ELISA, NEUT.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
The antibody is used for the neutralization of NGF in cell culture systems. Furthermore the antibody is used to immunosympathectomy.

Optimal working dilutions must be determined by end user.

Linkage

Replaces: 04-1119

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
Protein A purified

Storage and Stability

Maintain for 6 months at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Antibodies against mouse nerve growth factor interfere in vivo with the development of avian sensory and sympathetic neurones.
Rohrer, H, et al.
Development, 103, 545-552 (1988)
Reduced NGF secretion by Schwann cells under the high glucose condition decreases neurite outgrowth of DRG neurons.
Takahiro Tosaki,Hideki Kamiya,Yutaka Yasuda,Keiko Naruse,Koichi Kato,Mika Kozakae et al.
Experimental neurology null
Large-scale production of dsRNA and siRNA pools for RNA interference utilizing bacteriophage phi6 RNA-dependent RNA polymerase.
Aalto, AP; Sarin, LP; van Dijk, AA; Saarma, M; Poranen, MM; Arumae, U; Bamford, DH
RNA null
V Bocchini et al.
Proceedings of the National Academy of Sciences of the United States of America, 64(2), 787-794 (1969-10-01)
The nerve growth factor protein was purified over 100-fold from adult mouse salivary glands. The first step was a gel filtration on Sephadex G-100 at pH 7.5 of the aqueous gland extract. After gel filtration, most of the NGF activity
M Jakobson et al.
Cell death & disease, 4, e777-e777 (2013-08-24)
N-Bak is a neuron-specific BH3-only splice variant of pro-apoptotic Bcl-2 family member Bak. We have shown that its mRNA is stable in the neurons, whereas the protein cannot be detected by antibodies, suggesting a strong translational arrest of the mRNA.

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