MAB5272
Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
clone 324, Chemicon®, from rat
Synonym(s):
CD171, N-CAM L1
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
324, monoclonal
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... L1CAM(3897) , VCAM1(7412)
Related Categories
General description
Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts
Specificity
Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.
Immunogen
Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.
Application
This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
Western blotting:
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
Target description
120-140 kDa
Physical form
Format: Purified
Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellum.
Lindner, J, et al.
Nature, 305, 427-430 (1983)
Absence of layer-specific cadherin expression profiles in the neocortex of the reeler mutant mouse.
Hertel, N; Redies, C
Cerebral Cortex (1991)
Heparan sulfate sugar modifications mediate the functions of slits and other factors needed for mouse forebrain commissure development.
Conway, CD; Howe, KM; Nettleton, NK; Price, DJ; Mason, JO; Pratt, T
The Journal of Neuroscience null
Expression of ABCA2 protein in both non-myelin-forming and myelin-forming Schwann cells in the rodent peripheral nerve.
Takashi Saito, Katsuya Yamada, Yan Wang, Yukiko Tanaka, Kazuo Ohtomo, Kazuo Ishikawa, Nobuya Inagaki
Neuroscience Letters null
Neuron glia-related cell adhesion molecule (NrCAM) promotes topographic retinocollicular mapping.
Dai, J; Buhusi, M; Demyanenko, GP; Brennaman, LH; Hruska, M; Dalva, MB; Maness, PF
Testing null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service