MAB8419
Anti-Hepatitis B Virus Antibody, X-Protein, a.a. 90-115, clone 227
clone 227, Chemicon®, from mouse
Synonym(s):
Trans-Activator X Gene Product
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
227, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
shipped in
wet ice
General description
Protein X (UniProt: P69713; also known as HBx, Peptide X, pX) is encoded by the X gene (Gene ID: 944566) in Hepatitis B virus. Hepatitis B virus (HBV) contains seven main proteins, which include Core, pre-Core, Small S, Middle S, Large S, Polymerase, and the HBx protein. HBx, a multifunctional protein, although not essential for HBV infection, plays a role in silencing host antiviral defenses and promoting viral transcription. It facilitates the efficient replication of HBV by stimulating HBV gene expression from the cccDNA template. It is mainly localized in the cytoplasm with a small fraction detected in the nucleus. However, its expression level can also influence its cellular localization. It is predominantly nuclear when expressed in cells at very low levels but becomes largely cytoplasmic as its expression level increases. In cytoplasm, a minor fraction may also associate with mitochondria. Its mitochondrial targeting sequence is localized in amino acids 68-117. HBx has an N-terminal negative regulatory domain and its transactivation or coactivation of the C-terminal domain is reported to interfere with host cell signaling transduction pathways to help HBV replication. The best-characterized HBx binding partner is the cellular damage-specific DNA binding protein 1 (DDB1) and the interaction of HBx-DDB1 is essential for HBV replication. HBx promotes the degradation of the cellular structural maintenance of chromosomes 5/6 complex (Smc5/6), which directly binds DNA and entraps DNA plasmids. HBx is directly involved in development of cirrhosis and liver and hepatocellular carcinoma. (Ref.: Kornyeyev, D., et al. (2019). J. Virol. 93(16); e00248-19; Hwang, G-Y., et al. (2003). J. Clin. Microbiol. 41(12); 5598-5603).
Specificity
Reacts with Hbx-protein (trans-activator X gene product) of hepatitis B virus (epitope recognized - amino acid positions 90-115).
SPECIES REACTIVITIES:
Reacts only with HBV-infected human cells.
SPECIES REACTIVITIES:
Reacts only with HBV-infected human cells.
Immunogen
Epitope: X-Protein, aa 90-115
Peptide cooresponding to aa90-115 of h HBV-X protein.
Application
Anti-Hepatitis B Virus Antibody, X-Protein, a.a. 90-115, clone 227 detects level of Hepatitis B Virus & has been published & validated for use in ELISA, WB & IC.
ELISA, immunoblotting and immunocytochemistry staining of infected liver tissues and HBV-transfected hepatoma cells. Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Physical form
Format: Purified
Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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HBX causes cyclin D1 overexpression and development of breast cancer in transgenic animals that are heterozygous for p53.
Klein, A; Guhl, E; Tzeng, YJ; Fuhrhop, J; Levrero, M; Graessmann, M; Graessmann, A
Oncogene null
Accumulation of 8-hydroxy-2'-deoxyguanosine adducts in HBx recombinant HepG2 cells and HBx transgenic mice.
Ralph Gehrke, Maria A Brauchle, Kurt Reifenberg, Eberhard Hildt, Uwe Gruetzner et al.
Digestion null
Epigenetic modification induced by hepatitis B virus X protein via interaction with de novo DNA methyltransferase DNMT3A.
Da-Li Zheng, Li Zhang, Na Cheng, Xiao Xu, Qing Deng, Xiao-Mei Teng, Ke-Sheng Wang et al.
Journal of Hepatology null
T M Bui-Nguyen et al.
Oncogene, 29(8), 1179-1189 (2009-12-17)
Metastasis-associated protein 1 (MTA1), a master chromatin modifier, has been shown to regulate cancer progression and is widely upregulated in human cancer, including hepatitis B virus-associated hepatocellular carcinomas (HCCs). Here we provide evidence that hepatitis B virus transactivator protein HBx
KyeongJin Kim et al.
PloS one, 5(3), e8649-e8649 (2010-03-31)
Hepatitis B virus (HBV) is a major cause of chronic liver diseases, and frequently results in hepatitis, cirrhosis, and ultimately hepatocellular carcinoma. The role of HCV in associations with insulin signaling has been elucidated. However, the pathogenesis of HBV-associated insulin
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