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MABC153

Sigma-Aldrich

Anti-CKAP4 Antibody, clone 9C4.4

clone 9C4.4, from mouse

Synonym(s):

Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9C4.4, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_006816

UniProt accession no.

Q07065

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CKAP4(10970)

General description

Cytoskeleton-associated protein 4, also known as 63-kDa cytoskeleton-linking membrane protein or Climp-63 or p63, and encoded by the gene CKAP4, is an integral membrane protein that links the endoplasmic reticulum (ER) to microtubules. The binding of CKAP4 is dependent upon its phosphorylation state: dephosphorylated CKAP4 binds microtubules and phosphorylated CKAP4 does not bind microtubules. CKAP4 is localized to the endoplasmic reticulum membrane and plasma membrane; CKAP4 is also found in the cytoplasm and perinuclear regions upon APF stimulation. CKAP4 is expressed is a wide variety of cell types and tissues and CKAP4 appears to be critical for antibiotic cytotoxicity for certain drugs like Gentamycin which bind the drug and cause CKAP4 to dimerize and malfunction.

Immunogen

Epitope: coiled coil domain
GST-tagged recombinant protein corresponding to the coiled coil domain of human CKAP4.

Application

Detect Cytoskeleton-associated protein 4 using this mouse monoclonal antibody, Anti-CKAP4 Antibody & clone 9C4.4 validated for use in western blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected CKAP4 in 10 µg of HeLa cell lysate.

Target description

~ 65 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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