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MABC1794

Sigma-Aldrich

Anti-Sulf-2 Antibody, clone 5D5

Synonym(s):

Arylsulfatase, EC:3.1.6.13, Extracellular sulfatase Sulf-2, N-acetylglucosamine-6-sulfatase, hSulf-2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

5D5, monoclonal

mol wt

calculated mol wt 100 kDa
observed mol wt ~66 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 100

technique(s)

ELISA: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable

isotype

IgG2bκ

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

storage temp.

-10 to -25°C

Gene Information

human ... SULF2(55959)

Specificity

Clone 5D5 is a mouse monoclonal antibody that detects Sulf-2.

Immunogen

Full-length recombinant human Sulf-2.

Application

Quality Control Testing

Evaluated by Western Blotting with His/GST-tagged recombinant Human sulfatase 2 (Sulf-2).

Western Blotting Analysis: A 1:250 dilution of this antibody detected His/GST-tagged recombinant Human sulfatase 2 (Sulf-2).

Tested Applications

Inhibition Assay: A representative lot of this antibody inhibited the PDGFRβ-YAP pathway and cholangiocarcinoma (CCA) growth in a murine model. Luo, X., et al. (2021). Hepatology.;74(3):1411-1428.

Western Blotting Analysis: A representative lot detected Sulf-2 in Western Blotting applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).

Immunoprecipitation Analysis: A representative lot detected Sulf-2 in Immunoprecipitation applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).

ELISA: A representative lot detected Sulf-2 in ELISA applications (Singer, M.S., et al. (2015). Clin Chim Acta. 440:72-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Extracellular sulfatase Sulf-2 (UniProt: Q8IWU5; also known as hSulf-2, Arylsulfatase, EC:3.1.6.13, N-acetylglucosamine-6-sulfatase) is encoded by the SULF2 (also known as KIAA1247 and UNQ559/PRO1120) gene (Gene ID: 55959) in human. Sulfatase 1 (Sulf-1) and sulfatase 2 (Sulf-2) are heparan sulfatase editing sulfatases that remove 6-O sulfate moiety within heparan sulfate (HS) chains and alter the affinity of the HS chains for growth factors and growth factor receptors. Sulf-2 is synthesized with a signal peptide (aa 1-24) that is subsequently cleaved off to produce the mature protein. Its N-terminal region contains the catalytic domain (aa 1-415) that is followed by a hydrophilic domain (aa 416-715) and the C-terminal region. The hydrophilic domain is necessary for its endoglucosamine-6-sulfatase activity. Glycosylation at serine 583 is shown to negatively regulate its N-acetylglucosamine-6-sulfatase and arylsulfatase activities. Higher expression of Sulf-2 is reported in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney, and placenta. It is detected in the endoplasmic reticulum, Golgi apparatus, and on the cell surface. A secreted form has also been described that is produced after processing by furin. In a majority of hepatocellular carcinomas (HCC) Sulf-2 enhances tumor growth and progression via activation of the Wnt and TGF-β signaling. It has broad oncogenic effects in HCC, and it increase cell growth by upregulating glypican 3 that promotes release of FGF2 and Wnt form glypican 3 to bind to their cognate receptors and enhance receptor tyrosine kinase activity and Wnt/β-catenin signaling. Knockout of Sulf-2 is reported to suppress HCC tumorigenesis. Two isoforms of Sulf-2 have been described that are produced by alternative splicing. (Ref.: Luo, X., et al. (2021). Hepatology. 74(3); 1411-1428; Singer, MS., et al. (2015). Clin. Chim. Acta. 440; 72-78).

Physical form

Purified mouse monoclonal antibody IgG2b in PBS without azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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