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MABC605

Sigma-Aldrich

Anti-TXNIP Antibody, clone 3A7.1

clone 3A7.1, from mouse

Synonym(s):

Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

3A7.1, monoclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_006463

UniProt accession no.

Q9H3M7

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TXNIP(10628)

General description

Thioredoxin-interacting protein (UniProt Q9H3M7; also known as Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) is encoded by the TXNIP (also known as VDUP1) gene (Gene ID 10628) in human. Thioredoxin interacting protein (TXNIP) binds thioredoxin active cysteine residue and suppresses its antioxidant activity. TXNIP overexpression results in increased cellular ROS level and oxidative cell death, while TXNIP suppression enhances cellular resistance to oxidative injury. TXNIP is also shown to inhibit cellular glucose uptake by downregulating glucose transporter 1 (GLUT1) plasma membrane localization, and TXNIP expression is found to be markedly suppressed in many cancers, making TXNIP a potential anticancer therapeutic target.

Specificity

Clone 3A7.1 targets an epitope within the N-terminal half of TXNIP. Cross-reactivity to human and mouse spliced isoform 2 is likely, but has not been confirmed.

Immunogen

GST-tagged recombinant fragment from the N-terminal half of human TXNIP.

Application

Anti-TXNIP Antibody, clone 3A7.1 is an antibody against TXNIP for use in Western Blotting, Immunocytochemistry.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TXNIP in HUVECs, A431, HeLa, and NIH/3T3 cells.

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TXNIP in 10 µg of K562 cell lysate.

Target description

~44 kDa observed. 43.66/37.32 kDa (human isoform 1/2) and 44.36/44.29 kDa (mouse isoform 1/2) calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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