Anti-THAP11 Antibody, clone 3F3

THAP11 or Thanatos-associated protein 11, THAP domain-containing protein 11, encoded by the human gene named THAP11 or HRIHFB2206 is a sequence specific transcription factor critical for embryogenesis and the pluripotency of embryonic stem cells (ESCs). THAP11 is a transcriptional repressor that suppresses differentiation genes in ES cells by suppressing c-Myc. Overexpression of THAP11 markedly inhibits growth of a number of different cells, including some cancer cells and non-transformed cells. THAP11 is ubiquitously expressed in normal tissues and frequently downregulated in several human tumor tissues. Research demonstrates that among the cellular protein partners of THAP11 is a transcriptional activator PCBP1, where again overexpression inhibited CD44v6 expression (one of the homing receptors) and cell invasion in the cancer cell line HepG2. However THAP11 is not always associated with growth suppression as recent research in colon cancer indicates that THAP11 associates with the transcriptional coregulator HCF-1 (host cell factor 1) and recruits HCF-1 to target promoters leading to an increase in gene expression. Moreover THAP11 is found to be expressed in both primary tumors and metastases, and knockdown of THAP11 in SW620 colon cancer cells lines showed a marked decrease in cell proliferation and a down regulation of numerous genes by gene profiling. EMD-Millipore’s Anti-THAP11 mouse monoclonal antibody has been tested in western blot against HeLa and NTERA-2 cell lysates and paraffin embedded immunohistochemistry on colon and ovarian cancer tissue sections and in fluorescent immunocytochemistry on NTERA-2 cells in culture and in flow cytometry on HeLa cells in culture.

Purified recombinant fragment of human THAP11 expressed in E. Coli.

Anti-THAP11 Antibody, clone 3F3 is a highly specific mouse monoclonal antibody, that targets Thanatos-associated protein 11 & has been tested in western blotting, Immunofluorescence, IHC & Flow Cytometry.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected THAP11 in NTERA-2 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected THAP11 in paraffin-embedded colon cancer and ovarian tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected THAP11 in HeLa cells.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Evaluated by Western Blotting in HeLa and NTERA-2 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected THAP11 in HeLa and NTERA-2 cell lysates.

~48 kDa observed. Uncharacterized bands may appear in some lysate(s).

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa and NTERA-2 cell lysates

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.