Anti-CST complex subunit CTC1 Antibody, clone C482

CST complex subunit CTC1 (UniProt Q2NKJ3; also known as Conserved telomere maintenance component 1, HBV DNAPTP1-transactivated protein B) is encoded by the CTC1 (also known as C17orf68, CRMCC) gene (Gene ID 80169) in human. CTC1 is a subunit of the trimeric CST (CTC1, STN1, and TEN1) complex that binds ssDNA, promotes telomere DNA synthesis, and inhibits telomerase-mediated telomere elongation. CST directly interacts with and stimulates DNA polymerase -primase (pol -primase) activity. CST also associates with and sequesters telomeric 3′ overhangs after their elongation by telomerase, thereby limiting further telomere extension by telomerase. Knockdown of either STN1 or CCT1 in human cancer cells and in mouse fibroblasts results in substantially elongated telomeric G-ove+D13rhangs. Conditional deletion of CTC1 in mice leads to early entry into senescence and signs of telomeric replication defects, Human CTC1 gene mutations casue dyskeratosis congenita (DC) and related bone marrow failure syndromes, as well as the rare autosomal recessive neurological disorder Coats Plus, where patients develop cranial calcifications and cysts. Coats Plus and DC patients develop premature hair graying, anemia, and osteoporosis as a result of telomerase defects.

Clone C482 targets an N-terminal region sequence present in both human CTC1 spliced isoforms reported by UniProt (Q2NKJ3).

Linear peptide corresponding to an N-terminal region sequence of human CTC1.

Anti-CST complex subunit CTC1, clone C482, Cat. No. MABE1103, is a highly specific mouse monoclonal antibody that targets CTC1 and has been tested in and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 0.75-1 µg/mL from a representative lot detected the exogenously overexpressed CTC1 in 100-150 ng of lysate from transfected HeLa cells, as well as downregulated CTC1 expression following conditional CTC1 gene knockout in 150 ng of lysate from a HCT116 cell line harboring floxed CTC1 alleles (Courtesy of Carolyn Price, Ph.D. and Xuyang Feng, Ph.D., University of Cincinnati College of Medicine, U.S.A.).

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2b .

~135 kDa observed. 134.61/114.1 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified.

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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