MABE329
Anti-c-Fos Antibody, clone 2G9C3
clone 2G9C3, from mouse
Synonym(s):
Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2G9C3, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FOS(2353)
General description
c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.
Specificity
This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins.
Immunogen
Linear peptide corresponding to human c-Fos.
Application
Anti-c-Fos Antibody, clone 2G9C3 is a Mouse Monoclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB, ICC.
Immunocytochemistry Analysis: A representative lot detected c-Fos in schwann cells. (Lafarga, M., et al. (2002). Molecular Biology of the Cell. 13:2771–2782.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos on 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos on 10 µg of HeLa cell lysate.
Target description
~55/62 kDa observed. This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins. The calculated molecular wieght is 46 kDa; however, c-Fos has been observed at ~55 kDa in western blots. (Assim, M., et al. (2010). Journal of Biological Chemistry. 285(26):20343–20357.)
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ from cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mohammad Asim et al.
The Journal of biological chemistry, 285(26), 20343-20357 (2010-04-23)
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