MABE462
Anti-TET2 Antibody, clone hT2H 21F11
clone hT2H21F11, from mouse
Synonym(s):
Methylcytosine dioxygenase TET2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
hT2H21F11, monoclonal
species reactivity
human, mouse
technique(s)
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TET2(54790)
General description
Methylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS).
Specificity
This antibody recognizes the N-terminus of TET2
Immunogen
Recombinant protein corresponding to the N-terminus of human TET2.
Application
Detect Tet2 using this mouse monoclonal antibody, Anti-TET2 Antibody, clone hT2H 21F11 validated for use in Chromatin IP (ChIP), western blotting & IP.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected TET2 in 10 µg of TF-1 cell lysate.
Immunoprecipitation Analysis: 5 µg of a representative lot immunoprecipitated TET2 in 250 µg of HL60 RIPA cell lysate.
Immunoprecipitation Analysis: 5 µg of a representative lot immunoprecipitated TET2 in 250 µg of HL60 RIPA cell lysate.
Quality
Evaluated by Western Blotting in MOLT-4 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected TET2 in 10 µg of MOLT-4 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected TET2 in 10 µg of MOLT-4 cell lysate.
Target description
~ 260 kDa observed. The calculated molecular weight of this protein is 224 kDa, but can be observed at ~260 kDa
Physical form
Format: Purified
Analysis Note
Control
MOLT-4 cell lysate
MOLT-4 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Product No.
Description
Pricing
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lorenzo de la Rica et al.
Genome biology, 14(9), R99-R99 (2013-09-14)
DNA methylation is a key epigenetic mechanism for driving and stabilizing cell-fate decisions. Local deposition and removal of DNA methylation are tightly coupled with transcription factor binding, although the relationship varies with the specific differentiation process. Conversion of monocytes to
Yueyue Duan et al.
Journal of medical virology, 94(7), 3251-3256 (2022-02-26)
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered bat-origin coronavirus with fatal pathogenicity for neonatal piglets. There is no vaccine to prevent SADS-CoV infection or clinically approved drugs targeting SADS-CoV. Therefore, unraveling cellular factors that regulate SADS-CoV for
Yan-Ping Xu et al.
The Journal of clinical investigation, 130, 4316-4331 (2019-07-17)
Loss-of-function mutations in genes encoding TET DNA dioxygenase occur frequently in hematopoietic malignancy, but rarely in solid tumors which instead commonly have reduced activity. The impact of decreased TET activity in solid tumors is not known. Here we show that
Yundong He et al.
Nature communications, 12(1), 1521-1521 (2021-03-23)
Resistance to next-generation anti-androgen enzalutamide (ENZ) constitutes a major challenge for the treatment of castration-resistant prostate cancer (CRPC). By performing genome-wide ChIP-seq profiling in ENZ-resistant CRPC cells we identify a set of androgen receptor (AR) binding sites with increased AR
Eevi Kaasinen et al.
Nature communications, 10(1), 1252-1252 (2019-03-21)
Clonal hematopoiesis driven by somatic heterozygous TET2 loss is linked to malignant degeneration via consequent aberrant DNA methylation, and possibly to cardiovascular disease via increased cytokine and chemokine expression as reported in mice. Here, we discover a germline TET2 mutation
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