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MABE952

Sigma-Aldrich

Anti-Histone H3.1 Antibody, clone 1D4F2

clone 1D4F2, 1 mg/mL, from rat

Synonym(s):

Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1D4F2, monoclonal

species reactivity

human, mouse

concentration

1 mg/mL

technique(s)

ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_003520

UniProt accession no.

P68431

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HIST1H3F(8968)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3.1.

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Histone H3.1 in HeLa cells.

Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H3.1 in NIH/3T3 cells (Prof. Taro Tachibana, Cell Engineering Corporation.).

Alexa Fluor™ is a registered trademark of Life Technologies.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Histone H3.1 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).

Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).
This Anti-Histone H3.1 Antibody, clone 1D4F2 is validated for use in western blotting, ICC, IP, ChIP & ChIP-seq for the detection of Histone H3.1.

Quality

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.1 in 10 µg of HeLa acid extract.

Target description

~17 kDa observed

Physical form

Format: Purified

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples.
Maehara, Kazumitsu, et al.
Nucleic Acids Research, 41, 54-62 (2013)

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