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MABF147D

Sigma-Aldrich

Anti-Ly-6G (mouse), APC, clone RB6-8C5 Antibody

clone RB6-8C5, from rat, Allophycocyanin conjugate

Synonym(s):

Lymphocyte antigen 6G (Ly-6G), Ly-6G.1, Ly-6G

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

conjugate

Allophycocyanin conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

RB6-8C5, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG2bκ

UniProt accession no.

P35461

target post-translational modification

unmodified

Gene Information

mouse ... Ly6G(546644)

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General description

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

Application

This rat monoclonal Anti-Ly-6G Antibody (mouse), APC, clone RB6-8C5 , Cat. No. MABF1477 is validated for use in Flow Cytometry, for the detection of Ly-6G.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

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