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MABF2128

Sigma-Aldrich

Anti-Properdin Antibody, clone 6E11A4

clone 6E11A4, from mouse

Synonym(s):

Complement factor P

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6E11A4, monoclonal

species reactivity

baboon, rabbit, canine, human

packaging

antibody small pack of 25 μg

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001138724

UniProt accession no.

P27918

target post-translational modification

unmodified

Gene Information

human ... CFP(5199)

Related Categories

General description

Properdin (UniProt: P27918; also known as Complement factor P) is encoded by the CEP (also known as PFC) gene (Gene ID: 5199) in human. (ABF185: Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization.

Specificity

Clone 6E11A4 specifically detects human properdin.

Application

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Properdin in human tonsil tissue sections.

ELISA Analysis: A representative lot detected Properdin in ELISA applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).

Inhibition Analysis: A representative lot detected Properdin in Inhibition applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).
This mouse monoclonal Anti-Properdin, clone 6E11A4 Antibody, Cat. No. MABF2128 is tested for use in Western Blotting, ELISA, and Immunohistochemistry (Paraffin), for the detection of Properdin. Inhibits function in Properdin.

Quality

Evaluated by Western Blotting in purified human properdin.

Western Blotting Analysis: 1 µg/mL of this antibody detected Properdin in purified human properdin.

Target description

~51 kDa observed; 51.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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