Skip to Content
MilliporeSigma
All Photos(1)

Documents

MABF290

Sigma-Aldrich

Anti-S100A9 Antibody, clone 2A5 (Azide Free)

clone 2A5, 1 mg/mL, from rat

Synonym(s):

Protein S100-A9, Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, S100 calcium-binding protein A9

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2A5, monoclonal

species reactivity

human, mouse

concentration

1 mg/mL

technique(s)

activity assay: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NP_00126878

UniProt accession no.

P31725

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... S100A9(6280)

General description

S100A9, also known as Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, or S100 calcium-binding protein A9, and encoded by the gene S100A9/CAGB/MRP14/S100-A9 is a calcium and zinc binding protein that plays a prominent role in the regulation of inflammatory processes and immune responses. S100-A9 can induce neutrophil chemotaxis and adhesion. S100-A9 one of the subunits of Calprotectin, which is a complex of S100-A8 and S100-A9 proteins and is also critical for immune responses. S100-A9 is a cytoplasmic and secreted protein predominantly expressed in myeloid cells as well as neutrophils and monocytes but not expressed in lymphocytes. S100-A9 is found in high concentrations at sites of inflammation or in the serum of patients with inflammatory diseases. S100-A9 is also a cancer marker as it is up regulated in many cancers including gastric, esophageal, colon, breast and many more.

Immunogen

Recombinant protein corresponding to mouse S100A9.

Application

Activity Assay Analysis: A representative lot detected S100A9 and found that it increases neutrophil migration & induces the secretion of cytokines by monocytes (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e45478 1-12).
Western Blotting Analysis: A representative lot detected S100A9 in mouse neutrophils and recombinant mS100A9 (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e45478 1-12).
Anti-S100A9 Antibody, clone 2A5 (Azide Free) is an antibody against S100A9 for use in western blotting & activity assay.

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected S100A9 in 10 µg of Raji cell lysate.

Target description

~15 kDa observed

Physical form

Format: Purified
Purified rat monoclonal IgG1 in buffer containing PBS without preservatives.

Not finding the right product?  

Try our Product Selector Tool.

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

An inflammation loop orchestrated by S100A9 and calprotectin is critical for development of arthritis.
Cesaro, Annabelle, et al.
Testing, 7, e45478-e45478 (2012)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service