Anti-Eosinophil Cationic Protein Antibody, clone 1C8.5/D9

Purified human ECP.

Clone 1C8.5/D9 specifically reacted with ECP, but not other granule proteins by Western blotting analysis (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22).

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected eosinophil cationic protein in 50 µg of human liver and lung tissue lysates.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Eosinophil Cationic Protein in human small intestine and human colon tissue.
ELISA Analysis: A representative lot was employed as the capture antibody for the detection of purified human ECP, as well as ECP in serum samples from individuals with eosinophilic disorders by sandwich ELISA (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22),
Multiplexing Analysis: A representative lot was employed as the capture antibody in a bead-based multiplex assay for the detection of purified human ECP as well as ECP in serum samples from healthy donors and individuals with eosinophilic disorders. Prior reduction and alkylation of the serum samples (by DTT and iodoacetamide, respectively) resulted in higher EDN levels (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22)

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected eosinophil cationic protein in 50 µg of human spleen tissue lysate.

~20 kDa observed. Target band appears larger than the calculated molecular weights of 18.39 kDa (pro-form) and 15.52 kDa (mature) due to glycosylation. Uncharacterized band(s) may appear in some lysates.

Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

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