MABN845
Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41
clone PS 41, from mouse
Synonym(s):
Vasopressin-neurophysin 2-copeptin, AVP-NPII, Arg-vasopressin, Arginine-vasopressin, Neurophysin 2, Neurophysin-I, Copeptin, Neurophysin 2/NP-AVP
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PS 41, monoclonal
species reactivity
rat
technique(s)
ELISA: suitable
electron microscopy: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
rat ... Avp(24221)
Related Categories
General description
The neurophysin 1 and 2 (NPI and NPII) are carriers of peptide hormone oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone peptide are synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or AVP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI) or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. AVP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. AVP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
Specificity
Clone PS 41 reacts with Vasopressin-neurophysin 2-copeptin precursor, neurophysin 2, but not Arg-vasopressin, copeptin, oxytocin, or neurophysin 1.
Immunogen
Epitope: Neurophysin 2
KLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Neurophysin 2/NP-AVP in rat pituitary tissue.
Immunohistochemistry Analysis: A representative lot detected Neurophysin 2/NP-AVP immunoreactivity in the hypothalamus of developing rats as early as embryonic day (E16) using coronal vibratome sections. (Witnall, M.H., et al.(1985). J Neurosci. 5(1):98-109).
Radioimmunoassay Analysis: Target reactivity of a representative lot was confirmed by RIA using plates coated with purified rat Neurophysin/NP and 125I-labelled secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Electron Microscopy Analysis: A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunohistochemistry Analysis: A representative lot detected Neurophysin 2/NP-AVP immunoreactivity in the hypothalamus of developing rats as early as embryonic day (E16) using coronal vibratome sections. (Witnall, M.H., et al.(1985). J Neurosci. 5(1):98-109).
Radioimmunoassay Analysis: Target reactivity of a representative lot was confirmed by RIA using plates coated with purified rat Neurophysin/NP and 125I-labelled secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Electron Microscopy Analysis: A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
This Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41 is validated for use in Western Blotting and Immunohistochemistry, Radioimmunoassay, Electron Microscopy, Immunoprecipitation and ELISA for the detection of Neurophysin 2/NP-AVP.
Quality
Evaluated by Western Blotting in rat pituitary tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.
Target description
~15 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Neurophysin in the hypothalamo-neurohypophysial system. II. Immunocytochemical studies of the ontogeny of oxytocinergic and vasopressinergic neurons.
Whitnall, MH; Key, S; Ben-Barak, Y; Ozato, K; Gainer, H
The Journal of Neuroscience null
Mingfeng Zhou et al.
Neuroendocrinology (2021-11-12)
Hypothalamic injury causes several complicated neuroendocrine-associated disorders, such as water-electrolyte imbalance, obesity, and hypopituitarism. Among these, central diabetes insipidus (CDI), characterized by polyuria, polydipsia, low urine specific gravity, and deficiency of arginine vasopressin contents, is a typical complication after hypothalamic
Marie Habart et al.
STAR protocols, 4(1), 101968-101968 (2023-01-05)
Here, we present an optimized iDISCO+ protocol combining tissue clearing and light sheet microscopy to map the postnatal development of oxytocin and vasopressin neurons in mouse hypothalamus. We describe tissue preparation, immunostaining, clearing, and imaging. We then detail how to
Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies.
Ben-Barak, Y; Russell, JT; Whitnall, MH; Ozato, K; Gainer, H
The Journal of Neuroscience null
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