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MABS26

Sigma-Aldrich

Anti-LDLR Antibody, clone C7

clone C7, from mouse

Synonym(s):

low density lipoprotein receptor, LDL receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

C7, monoclonal

species reactivity

human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology)

technique(s)

western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001160002

UniProt accession no.

P01131

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LDLR(3949)

General description

Low-Density Lipoprotein (LDL) Receptor is a mosaic protein that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). Mutations in LDLR are associated with familial hypercholesterolemia which is characterized by high levels of LDL in the blood.

Specificity

This antibody recognizes LDLR.

Immunogen

Partially purified bovine adrenal LDL receptor

Application

Research Category
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
This Anti-LDLR Antibody, clone C7 is validated for use in WB for the detection of LDLR.

Quality

Evaluated by Western Blot in ARPE-19 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LDLR on 10 µg of ARPE-19 cell lysate.

Target description

160 kDa Observed The translation of LDLR mRNA yields a 120 kDa protein that is post-translationally modified in the Golgi apparatus into the mature, 160 kDa LDLR. (Tolleshaug, H., et al. (1982). Cell 30:715–724.)

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
ARPE-19 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yushan Wang et al.
Frontiers in cell and developmental biology, 8, 580599-580599 (2020-12-18)
Abnormality in cholesterol homeostasis in the brain is a feature of Alzheimer's disease (AD). 27-Hydroxycholesterol (27-OHC) has been identified as a possible biomarker of AD, but its effects on cholesterol metabolism have not been fully characterized. This study was aimed

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