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MABS430

Sigma-Aldrich

Anti-Mcpip1, clone 2H8.2 Antibody

clone 2H8.2, from mouse

Synonym(s):

Mcpip1, Ribonuclease ZC3H12A, MCP-induced protein 1, Zinc finger CCCH domain-containing protein 12A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2H8.2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_079355

UniProt accession no.

Q5D1E8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ZC3H12A(80149)

General description

MCP-induced protein 1 (also known as ribonuclease ZC3H12A, Zinc finger CCCH domain-containing protein 12A) is encoded by the ZC3H12A gene in human (also known as dJ423B22.1, MCPIP, MCPIP1). It belongs to the 4-member CCCH-zinc finger protein family (MCPIP1/2/3/4) that act as negative regulators in macrophage activation (PMID 18178554). MCPIP1 in particular is involved in immune response downregulation upon LPS treatment by targeting IL-6 and IL-12b with its RNase activity (PMID 22561375). MCPIP1 expresssion by MCP-1 treatment is reported to promote angiogenesis in human umbilical vein endothelial cell (HUVEC) cultures, while causing apoptotic cell death in cardiac myocytes (PMID 18364357 & 16574901).

Immunogen

GST-tagged recombinant protein corresponding to human Mcpip1.

Application

Anti-Mcpip1, clone 2H8.2 Antibody is an antibody against Mcpip1 for use in Immunohistochemistry and Immunocytochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MCPIP1 in human kidney tissue.

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MCPIP1 in 10 µg of THP-1 cell lysate.

Target description

~65 kDa observed

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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