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MABS518

Sigma-Aldrich

Anti-CBS Antibody, clone 9F3.2

clone 9F3.2, from mouse

Synonym(s):

Cystathionine beta-synthase, CBS, Beta-thionase, Serine sulfhydrase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9F3.2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001171479

UniProt accession no.

P35520

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CBS(875)

General description

Cystathionine beta-synthase (EC 4.2.1.22; UniProt P35520; also known as beta-thionase, Methylcysteine synthase, Serine sulfhydrase) is encoded by the CBS (also known as HIP4) gene (Gene ID 875) in human. Cystathionine beta-synthase catalyzes the trans-sulfuration of homocysteine and serine to form the cysteine precursor cystathionine in the folate pathway. Cysteine is an essential substrate for the biosynthesis of glutathione, a major endogenous antioxidant. CBS deficiency results in altered sulfur metabolism and homocystinuria characterized by increased urinary homocystine and methionine. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events.

Specificity

Expected to react with both the Major (isoform 1) and the Minor (isoform 2) spliced variant.

Immunogen

Recombinant protein corresponding to the N-terminal of human CBS.

Application

Anti-CBS Antibody, clone 9F3.2 is an antibody against CBS for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CBS in human liver and human pancreas tissue.

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CBS in 10 µg of Raji cell lysate.

Target description

~61 kDa observed

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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