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MABT100

Sigma-Aldrich

Anti-Girdin Antibody, CT, clone 10E6.1

clone 10E6.1, from mouse

Synonym(s):

Girdin, Akt phosphorylation enhancer, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, Girders of actin filament, Hook-related protein 1, HkRP1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10E6.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001129069

UniProt accession no.

Q3V6T2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CCDC88A(55704)

General description

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

Immunogen

Recombinant protein corresponding to human Girdin.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Girdin in epithelial cells of human prostate tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in COS-7 cell lysate.
This Anti-Girdin, C-terminus Antibody, clone 10E6.1 is validated for use in Western Blotting, IHC(P), ICC, Immunoprecipitation for the detection of Girdin, C-terminus, clone 10E6.1.

Quality

Evaluated by Western Blot in human brain tissue lysate.

Wesetern Blot Analysis: A 1:5000 dilution of this antibody detected Girdin in 10 µg of human brain tissue lysate.

Target description

~216 kDa observed

Physical form

Format: Purified

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Aki Tanouchi et al.
Journal of experimental & clinical cancer research : CR, 35(1), 190-190 (2016-12-07)
Coiled-Coil Domain Containing 88A (CCDC88A) was identified as a substrate of the serine/threonine kinase Akt that is capable of binding to the actin cytoskeleton. The aim of this study was to investigate the potential role of CCDC88A in the migration

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