Skip to Content
MilliporeSigma
All Photos(1)

Documents

MABT165

Sigma-Aldrich

Anti-p130cas/BCAR1 Antibody, clone 2C1.1

clone 2C1.1, from mouse

Synonym(s):

Breast cancer anti-estrogen resistance protein 1, Cas scaffolding protein family member 1, Crk-associated substrate, p130Cas

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2C1.1, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgMκ

NCBI accession no.

NP_055382

UniProt accession no.

P56945

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... BCAR1(9564)

General description

Breast cancer anti-estrogen resistance protein 1 (UniProt P56945; also known as Cas scaffolding protein family member 1, Crk-associated substrate, p130Cas) is encoded by the BCAR1 (also known as CAS, CAS1, CASS1, CRKAS) gene (Gene ID 9564) in human. p130Cas/BCAR1 is an adaptor protein that belongs to the CAS family of scaffold proteins, members of which also include Nedd9, HEF-1 (CAs-L), EFS, and CASS4. Structurally, p130Cas is composed of a Src-homology 3 (SH3) domain, an adjacent large substrate-binding domain containing 15 repetitions of the YxxP motif, a main site of tyrosine phosphorylation on the p130Cas/BCAR1 molecule that generates SH2-binding sites, a proline and serine rich region, and a highly conserved four-helix bundle (focal adhesion targeting [FAT] domain). p130Cas/BCAR1 plays an important role in transducing signaling originated from mechanical stimuli, extracellular matrix (ECM) adhesion, as well as growth factor stimulation cascades during normal development and diseases.

Specificity

Expected to react with all eight spliced isoforms of human p130Cas reported by UniProt (P56945-1 through P56945-8).

Immunogen

GST-tagged recombinant human p130cas/BCAR1 C-terminal fragment.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected p130cas/BCAR1 in 10 µg of A431 cell lysate.

Target description

~93 kDa observed. 93.14-97.88 kDa (isoforms 1-3 and isoforms 5-8) and 77.73 kDa (isoform 4) calculated. Fully phosphorylated target band migrates with an apparent molecular size of ~130 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Not finding the right product?  

Try our Product Selector Tool.

wgk_germany

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service