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MABT372

Sigma-Aldrich

Anti-Mastl Antibody, clone 4F9

clone 4F9, from mouse

Synonym(s):

Serine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

4F9, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001165774

UniProt accession no.

Q96GX5

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MASTL(84930)

Related Categories

General description

Mastl is also called Serine/threonine-protein kinase greatwall (GW) and Microtubule-associated serine/threonine-protein kinase-like (MAST-L). Mastl is a serine/threonine kinase that regulates mitosis entry and maintenance during M phase through the inactivation of protein phosphatase 2A (PP2A). Mastl is also thought to be involved in megakaryocyte differentiation and the diseaseThrombocytopenia 2 (THC2).

Immunogen

Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of human Mastl.

Application

Detect Mastl using this mouse monoclonal antibody, Anti-Mastl Antibody, clone 4F9 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected Mastl in human prostate cancer and human testis tissue sections.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting in human oral squamos cell carcinoma lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Mastl in 10 µg of human oral squamos cell carcinoma lysate.

Target description

~97 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Belén Sanz-Castillo et al.
The EMBO journal, 42(2), e110833-e110833 (2022-11-11)
The AKT-mTOR pathway is a central regulator of cell growth and metabolism. Upon sustained mTOR activity, AKT activity is attenuated by a feedback loop that restrains upstream signaling. However, how cells control the signals that limit AKT activity is not

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