MABT400
Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody
clone 5C11, from mouse
Synonym(s):
Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
5C11, monoclonal
species reactivity
canine, bovine, goat, rat, porcine, human, guinea pig
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PRG4(10216)
General description
The protein called Lubricin/Proteoglycan 4 is an important secreted protein in the joint critical for joint function and thus is also a focus of research in joint diseases as well. Lubricin/Proteoglycan 4 plays a role in boundary lubrication and prevents the deposition of cartilage from the synovial fluid by controlling the synovial growth of the synovial cells and inhibiting the adhesion of the cells to the cartilage surface. During development, Lubricin/Proteoglycan 4 also acts as a growth factor for hematopoietic and endothelial cell lineages, and Lubricin/Proteoglycan 4 appears important for proper barrier and lubrication development in tissues like the eye. Lubricin/Proteoglycan 4 is highly expressed in joints and near joint boundaries but can also be found in the brain, heart, lung, kidney and other tissues. Lubricin/Proteoglycan 4 has several isoforms that differ in their cell and tissue expression. Isoform C and D are the most widely expressed forms. Finally because of its effects on surfaces, Lubricin/Proteoglycan 4 is being examined in tissue engineering and artificial cartilage development as a treatment for joint diseases.
Immunogen
Recombinant protein corresponding to human Lubricin/Proteoglycan 4.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lubricin/Proteoglycan 4 in human liver and human heart tissue.
Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
Western Blotting Analysis: A representative lot detected purified bovine synovial lubricin from calf and steer under both reduced and non-reduced conditions (Schmidt, T.A., et al. (2009). Biochim Biophys Acta. 1790(5):375-384).
Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
Western Blotting Analysis: A representative lot detected purified bovine synovial lubricin from calf and steer under both reduced and non-reduced conditions (Schmidt, T.A., et al. (2009). Biochim Biophys Acta. 1790(5):375-384).
This Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Lubricin/Proteoglycan 4.
Quality
Evaluated by Western Blotting in Lubercin purified protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.
Target description
~220 kDa observed. The calculated molecular weight of this protein is 151 kDa with many isoforms observed around 145 kDa and it can run higher due to glycosylation.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Disulfide-bonded multimers of proteoglycan 4 PRG4 are present in normal synovial fluids.
Schmidt, TA; Plaas, AH; Sandy, JD
Biochimica et Biophysica Acta null
David C Browe et al.
Materials today. Bio, 16, 100343-100343 (2022-07-23)
Articular cartilage defects fail to heal spontaneously, typically progressing to osteoarthritis. Bone marrow stimulation techniques such as microfracture (MFX) are the current surgical standard of care; however MFX typically produces an inferior fibro-cartilaginous tissue which provides only temporary symptomatic relief.
Guido Carpino et al.
Clinical proteomics, 16, 37-37 (2019-11-07)
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