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MADPNMAG-70K-01

Millipore

MILLIPLEX® Mouse Adiponectin Magnetic Bead- Single Plex - Endocrine Assay

This MILLIPLEX MAP Mouse Adiponectin Magnetic Bead Single Plex Kit is an Endocrine single plex assay. The analyte available for this kit is Adiponectin.

Synonym(s):

Adiponectin Assay Kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 96%
sensitivity: 3.0 pg/mL
standard curve range: 12.2-50,000 pg/mL
inter-assay cv: <20%
intra-assay cv: <15%

technique(s)

multiplexing: suitable

compatibility

configured for Single Plex

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Adipocytes secrete several hormones called adipokines with functions that include appetite and energy balance and insulin sensitivity. One of these, adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Its anti-inflammatory effects on the cellular lining of blood vessel walls may explain the association of high adiponectin levels with reduced risk of heart attack.

MILLIPLEX® Mouse Adiponectin Single Plex Kit is to be used for the simultaneous quantification of Adiponectin in mouse serum, plasma, tissue/cell lysate and culture supernatant samples. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificity

Cross Reactivty
Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.

Application

  • Analyte: Adiponectin
  • Recommended Sample Type: Mouse serum, plasma, cell/tissue culture supernatants and lysates
  • Recommended Sample Dilution: 25 μL per well of 1:5000 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C).
  • Research Category: Endocrine
  • Research Subcategory: Obesity, Metabolic Disorders

Packaging

Everything you need in a single kit.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

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WGK 3


Certificates of Analysis (COA)

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Malgorzata E Skaznik-Wikiel et al.
Biology of reproduction, 94(5), 108-108 (2016-04-01)
Excess calorie consumption, particularly of a diet high in fat, is a risk factor for both obesity and reproductive disorders. Animal model studies indicate that elevated dietary fat can influence some reproductive functions independent of obesity. In the current study
Benjamin Korman et al.
Arthritis research & therapy, 20(1), 145-145 (2018-07-13)
A pivotal role for adipose tissue homeostasis in systemic sclerosis (SSc) skin fibrosis is increasingly recognized. The nuclear receptor PPAR-γ is the master regulator of adipogenesis. Peroxisome proliferator activated receptor-γ (PPAR-γ) has antifibrotic effects by blocking transforming growth factor-β (TGF-β)
Shotaro Kamata et al.
FEBS open bio, 8(9), 1524-1543 (2018-09-07)
Overnight fasting is a routine procedure before surgery in clinical settings. Intermittent fasting is the most common diet/fitness trend implemented for weight loss and the treatment of lifestyle-related diseases. In either setting, the effects not directly related to parameters of
Eun Wha Choi et al.
Scientific reports, 10(1), 12001-12001 (2020-07-21)
Brown adipose tissue generates heat via the mitochondrial uncoupling protein UCP1 to protect against obesity and hypothermia. Fas mutant MRL/lpr mice exhibit a significantly leaner phenotype compared to wild type MRL/MpJ mice. In this study, we evaluated the inflammatory cell
Feng Zhang et al.
iScience, 24(12), 103382-103382 (2021-11-30)
GLP-1 analogs are a class of glucose-lowering agents with multiple benefits in diabetes, but its role in adipose tissues remains to be elucidated. The aim of this study was to determine the action of recombinant human GLP-1 (rhGLP-1) Beinaglutide (BN)

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Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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