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MGAMMAG-300K

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MILLIPLEX® Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in murine serum, plasma and cell culture samples.

Synonym(s):

Luminex® Mouse Isotyping Assay, Millipore Mouse Isotyping Assay, Mouse IgG, IgA, IgM immunoassay, Mouse Isotyoing Multiplex Assay

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 100%
(IgM)
accuracy: 85%
(IgG3)
accuracy: 98%
(IgA)
accuracy: 98%
(IgG2b)
sensitivity: 0.18 ng/mL
(IgG2b)
sensitivity: 0.32 ng/mL
(IgG3)
sensitivity: 0.67 ng/mL
(IgG2a)
sensitivity: 0.7 ng/mL
(IgG1)
sensitivity: 1.1 ng/mL
(IgA)
sensitivity: 1.73 ng/mL
(IgM)
standard curve range: 1-900 ng/mL
(IgG2b)
standard curve range: 2-1,500 ng/mL
(IgG1)
standard curve range: 2-1,500 ng/mL
(IgG3)
standard curve range: 2-1,500 ng/mL
(IgM)
standard curve range: 3-2,000 ng/mL
(IgG2a)
standard curve range: 5-3,000 ng/mL
(IgA)
inter-assay cv: 14-17%
intra-assay cv: 5-10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens, and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Six major isotypes have been identified in placental mammals: IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions, and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes.

MILLIPLEX® Mouse Isotyping Bead Panel, measuring mouse IgG subclasses (1, 2a, 2b, 3), IgM, and IgA, has been designed to enable you to measure both classes and subclasses accurately – all in one well. In addition, this panel allows you to choose only those immunoglobulins you need for your experiments.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Application

  • Analytes: IgG1, IgG2a, IgG2b, IgG3, IgA, IgM
  • Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant
  • Recommended Sample dilution: 1:50,000 diluted plasma or serum, or cell culture supernatant diluted to approximately 1μg/mL Ig well.
  • Assay Run Time: One day
  • Research Category: Immunology and Inflammation, Isotyping

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Linkage

Replaces: MGAM-300

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

wgk_germany

WGK 3

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Tao Huang et al.
JCI insight, 1(7), e86689-e86689 (2016-10-05)
Eosinophilic inflammation and Th2 cytokine production are central to the pathogenesis of asthma. Agents that target either eosinophils or single Th2 cytokines have shown benefits in subsets of biomarker-positive patients. More broadly effective treatment or disease-modifying effects may be achieved
Common tolerance mechanisms, but distinct cross-reactivities associated with gp41 and lipids, limit production of HIV-1 broad neutralizing antibodies 2F5 and 4E10.
Chen, Y; Zhang, J; Hwang, KK; Bouton-Verville, H; Xia, SM; Newman, A; Ouyang, YB; Haynes et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Jianhui Wang et al.
Oncotarget, 7(19), 26949-26965 (2016-04-23)
Although there were considerable advances in the anti-aging medical field, it is short of therapeutic drug for anti-aging. Mounting evidence indicates that the immunosenescence is the key physiopathological mechanism of aging. This study showed the treatment of LW-AFC, an herbal
Haiqiang Yang et al.
Viruses, 14(3) (2022-03-27)
Coronavirus disease 2019 (COVID-19) has caused massive health and economic disasters worldwide. Although several vaccines have effectively slowed the spread of the virus, their long-term protection and effectiveness against viral variants are still uncertain. To address these potential shortcomings, this
Yogesh Saini et al.
Physiological reports, 6(8), e13677-e13677 (2018-04-19)
Macrophages (MΦ) are key sentinels of respiratory exposure to inhaled environmental stimuli. In normal "healthy" tissues, MΦ are believed to be a dormant cell type that, upon exposure to stress-causing stimuli, may get activated to exhibit pro- or anti-inflammatory roles.
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Immunology Multiplex Assays | MILLIPLEX® Assays

Immunology multiplex assays, like MILLIPLEX® assays, allow researchers to investigate more immune biomarkers simultaneously, saving time and resources. This is especially critical to immunology research, where numerous analytes play a role in different pathways.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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