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MHA000P2E

Millipore

m-Endo Total Coliform Broth

ready-to-use, ampule of 2 mL, suitable for microbiology, for coliforms

Synonym(s):

MF Endo Medium, m-Coliform Broth

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About This Item

UNSPSC Code:
41106225
eCl@ss:
32160801
NACRES:
NA.74

form

liquid

Quality Level

100

shelf life

12 mo.

feature

ready-to-use

packaging

ampule of 2 mL
pack of 50 tests

color

pink to light purple, with slight precipitate

input

sample type wine
sample type cosmetics
sample type food(s)
sample type beverage(s)
sample type cider
sample type sport drink
sample type bottled water
sample type soft drink
sample type beer

pH

7.2±0.2 (25.0 °C)

application(s)

bioburden testing
clinical
cosmetics
environmental
food and beverages
industrial qc
water monitoring
microbiology

compatibility

for use with EZ-Fit® Filtration Manifold
for use with MF-method
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

Escherichia spp.
coliforms

Related Categories

General description

m-Endo Total Coliform Broth is a selective and differential medium used to estimate the coliform bacteria in water samples by membrane filtration technique. The medium enables differentiation between lactose fermenters and non-fermenters. The use of sodium sulfite and basic fuchsin instead of bile salts inhibits the growth of Gram-positive bacteria. Lactose fermenting bacteria produce acetaldehyde that reacts with the sodium sulfite and fuchsin to form red colonies. The rapid fermentation of lactose by the organisms produces aldehydes, which leads to the development of a metallic sheen. Lactose non-fermenters form colorless colonies.

Application

m-Endo Total Coliform Broth is suitable for Total coliform detection in water samples. The media enables to differentiate between lactose fermenters from lactose non-fermenters.

Features and Benefits

  • Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
  • They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Specifications

Incubation Temperature: 35 °C ± 0.5
Incubation Time: 24 hrs ± 2
Standard Methods for the Examination of Water and Wastewater

Analysis Note

Coliform colonies appear deep red with distinct green metallic sheen
QC organisms
E. coli (ATCC 25922)

Mixed culture (E. coli, P. aeruginosa and P. vulgaris)
Target Microorganisms: Total coliforms

Legal Information

EZ-FIT is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Improved membrane filter medium for the detection of coliform organisms
J. Amer. Water Works Assoc., 50(2), 193-196 (1958)
Manafi M
Handbook of Culture Media for Food and Water Microbiology, 50(2), 233-233 (2011)

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