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OP80

Sigma-Aldrich

Anti-APC (Ab-7) Mouse mAb (CC-1)

Synonym(s):

Anti-Adenomatus Polyposis Coli

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

clone

CC-1, monoclonal

form

liquid

contains

≤0.1% sodium azide

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (frozen sections): suitable

dilution

(Free-floating SectionsParaffin Sections)

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

General description

Recognizes APC in oligodendrocytes and astrocytes.

  • Antibody Target Gene Symbol: APC
  • Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
  • Entrez Gene Name: adenomatous polyposis coli
  • Hu Entrez ID: 324 (Related Antibodies: ST1150, OP62, OP47L, OP44)
  • Mu Entrez ID: 11789
  • RatEntrez ID: 24205

Immunogen

a recombinant protein consisting of amino acids 1-226 of APC

Application

Frozen Sections (5 µg/ml) Immunofluorescence (5 µg/ml) Paraffin Sections (5 µg/ml, heat pre-treament required) Immunocytochemistry (see application references) Free Floating Sections (see application references)

Well suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody′s staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration

Warning

Toxicity: Standard Handling

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative control: MOPC 21 cells

Positive control: Purkinje cells or cerebellum tissue

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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