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PRCY2MG40PMX25BK

Millipore

MILLIPLEX® Non-Human Primate Cytokine/Chemokine Panel II - 25-Plex Premixed Space Saver (Bulk) Packaging

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple cytokine and chemokine biomarkers in non-human primate serum, plasma and cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

nonhuman primates

manufacturer/tradename

Milliplex®

assay range

standard curve range: 1-1,500 pg/mL
(IL-17A)
standard curve range: 1-750 pg/mL
(RANTES/CCL5 (tissue culture))
standard curve range: 10-10,000 pg/mL
(sCD137, IL-2, IP-10/CXCL10)
standard curve range: 146-150,000 pg/mL
(IL-4)
standard curve range: 15-15,000 pg/mL
(IL-21, IL-31)
standard curve range: 20-20,000 pg/mL
(FGF-2, IL-33)
standard curve range: 24-25,000 pg/mL
(IL-23)
standard curve range: 29-30,000 pg/mL
(IL-1α, IL-28A)
standard curve range: 293-300,000 pg/mL
(IL-17E/IL-25)
standard curve range: 3-3,000 pg/mL
(Eotaxin/CCL11, IL-6)
standard curve range: 4-4,000 pg/mL
(Granzyme B)
standard curve range: 7-7,000 pg/mL
(MIP-3α/CCL20)
standard curve range: 73-75,000 pg/mL
(IL-16)
standard curve range: 78-80,000 pg/mL
(Granzyme A)
standard curve range: 8-8,000 pg/mL
(sFasL)
standard curve range: 98-100,000 pg/mL
(Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA)

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.

The MILLIPLEX® Non-Human Primate Premixed Cytokine Panel II is a 25 plex kit to be used for the simultaneous quantification of the following analytes
in monkey serum, plasma, tissue/cell lysate and culture supernatant samples: sCD137, Eotaxin, sFASL, FGF-2, Fractalkine, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, MIP-3α, Perforin, RANTES and TNFβ.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
This multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details.
UPDATE >>
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, RANTES/CCL5, TNFβ/LTA
  • Recommended Sample type: Serum, plasma, or cell culture supernatant
  • Recommended Sample dilution: 1:2 diluted plasma or serum
  • NOTE: RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs
  • Assay Run Time: Overnight or one day. For best results, an overnight incubation is recommended
  • Research Category: Inflammation & Immunology

Components

Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized

Sample Plate, 96 well format, 1 plate

2 Foil plate sealers

Assay Buffer, 2 bottles, 30 mL

Bead Diluent, 1 bottle, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL

Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Premixed 24-plex Beads, 1 bottle, 3.5 mL

RANTES beads, 1 vial

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

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