CpGenome Rat Methylated Genomic DNA Standard

The CpGenome Rat Methylated Genomic DNA Control is an ideal control or standard in DNA methylation studies. This methylated standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase followed by a treatment with a CpG Methylase. This process results in a DNA standard which contains ≥ 95% methylated cytosines across the genome in both heterochromatic and euchromatic regions.

Research Category
Epigenetics & Nuclear Function

Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.

Quantitative Methylation Specific PCR (qMSP):
Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.

5 µg DNA in buffer containing 10mM Tris-HCl pH 8.0, 0.1mM EDTA

Upon receipt aliqout and store at -20°C to avoid multiple freeze thaws which can fragment DNA. For best quality, limit freeze and thaw of the same –20°C DNA aliquot to no more than 3 times. For longer term storage, the DNA can be stored at -80°C. With proper storage and protection from excess freeze-thaw cycles, this product is stable for up to two years from the date of receipt.

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