SHSY-5Y Tet-On Inducible Alpha-Synuclein Neuroblastoma Cell Line

The SHSY-5Y Tet-ON inducible α-synuclein neuroblastoma cell line serves as a model for both dopaminergic neuronal biology and α-synuclein pathology. SHSY-5Y cells may be induced upon treatment with retinoic acid to differentiate into neuronal-type cells, enabling studies of neuronal function and differentiation.4 SHSY-5Y cells secrete catecholamines and thus serve as a model for dopaminergic neurons. The SHSY-5Y TetON α-synuclein inducible cell line harbors an α-synuclein construct inducible by treatment with doxycycline, thus allowing controlled overexpression of α-synuclein before or after neuronal differentiation.5 The construct also includes a tet repressor, which prevents leaky expression in absence of inducer. This unique cell line is an excellent model for both the basic cellular physiology of Parkinson′s disease and therapeutic development.

Parkinson′s disease, the most common neurodegenerative movement disorder1, is caused by the progressive degeneration of dopaminergic neurons. A signature pathology of the brains of Parkinson′s patients is the buildup of misfolded aggregates of the protein α-synuclein.2 Aggregates of α-synuclein are also implicated in Lewy body dementia and Alzheimer′s disease.3 Although study of the complete pathogenesis of neurodegenerative diseases such as Parkinson′s requires animal models, cellular models that reflect distinct aspects of Parkinson′s pathology are of great value for understanding the multifaceted character of neurodegenerative diseases and useful for developing targeted treatments.The SHSY-5Y Tet-ON inducible α-synuclein neuroblastoma cell line serves as a model for both dopaminergic neuronal biology and α-synuclein pathology. SHSY-5Y cells may be induced upon treatment with retinoic acid to differentiate into neuronal-type cells, enabling studies of neuronal function and differentiation.4 SHSY-5Y cells secrete catecholamines and thus serve as a model for dopaminergic neurons. The SHSY-5Y TetON α-synuclein inducible cell line harbors an α-synuclein construct inducible by treatment with doxycycline, thus allowing controlled overexpression of α-synuclein before or after neuronal differentiation.5 The construct also includes a tet repressor, which prevents leaky expression in absence of inducer. This unique cell line is an excellent model for both the basic cellular physiology of Parkinson′s disease and therapeutic development.Source The SHSY-5Y Tet-ON Inducible -Synuclein cell line is stably transfected with a construct containing an alpha-synuclein cDNA cassette and CMV promoter under control of Tet-ON response element.5 The parental SHSY-5Y cell line was derived from a metastatic neuroblastoma of a 4-year-old female.6References1. Falkenburger BH, Saridaki T, Dinter E. J Neurochem 2016;139 (Suppl 1):121-130.2. Balestrino R, Schapira AHV. Eur J Neurol 2020; 27(1):27-42.3. Twohig D, Nielsen HM. Mol Neurodegener 2019; 14(1):23. 4. Agholme L et al. J Alzheimer′s Dis 2010; 20(4):1069-1082.5. Vasquez V, Mitra J, Perry G, Rao KS, Hegde ML. J Alzheimer′s Dis 2018; 66(2):453-460.6. Biedler JL, Helson L, Spengler BA. Cancer Res 1973; 33(11):2643-2652.

• Each vial contains ≥ 1X10⁶ viable cells.• SHSY-5Y Tet-ON Inducible -Synuclein Neuroblastoma cells are verified to be of human origin and negative for mouse, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services • Cells tested negative for infectious diseases against a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.• Cells tested negative for mycoplasma.

SHSY-5Y Tet-ON inducible α-synuclein neuroblastoma cell line serves as a model for both dopaminergic neuronal biology and α-synuclein pathology.

The cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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