T24T Human Bladder Carcinoma Cell Line

Bladder cancer originates from the epithelium that covers the inner surface of the bladder. The most common type is urothelial or transitional carcinoma (1). Most of the newly diagnosed cases (75%) are non-invasive, if the tumor becomes invasive it is more likely to metastasize to lymph nodes and other organs. Two common alterations have been identified in non-invasive tumors, deletion of chromosome 9 and a point mutation in FGFR3 (Fibroblast growth factor receptor 3) (2). Activating mutations in RAS gene family members are also common in bladder carcinoma and it is estimated that activation of the RAS-MAPK pathway may contribute to over 80% of the non-invasive cases (1). Loss of function of tumor suppressor genes like TP53 and RB1 are common in cases of invasive disease (1). After diagnosis the patient requires resection of the tumor and in cases of invasive disease chemotherapy is also provided.The original cell line T24 has been used to study bladder tumor biology but has some limitations because it lacks tumorigenicity when injected into immunocompromised mice. In contrast, T24T has increased metastatic ability when tested in vivo and expresses higher levels of HRAS mRNA (3). Increased HRAS activity leads to focal adhesion disassembly and los of CDH1 and b-catenin from intercellular junctions (3). Contact inhibition growth is reduced in T24T compared with T24 (3). Additionally, T24T does not inhibit its motile activity at higher density like T24 does.T24 cell line was generated from an invasive human transitional cell carcinoma of the bladder (4). T24T is a derivative of T24.1. Nat Rev Dis Primers 2017, 3: 17022.2. Int J Cancer 2006, 119(5): 1212-1215.3. Genes Chromosomes Cancer 2000, 27(3): 252-263.4. Int J Cancer 1973, 11(3): 765-773.

Each vial contains ≥1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells are negative for mycoplasma contamination.

T24T human bladder cancer cell line is a useful model for the study of metastatic bladder cancer.

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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