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Sigma-Aldrich

BioTracker MemBright 640 Live Cell Dye

Synonym(s):

Live cell imaging probe, Live cell membrane probe, far red

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About This Item

UNSPSC Code:
41106514

mol wt

observed mol wt 1396.4 kDa

Quality Level

packaging

vial of 50 μL

manufacturer/tradename

Millipore

growth mode

N/A

technique(s)

cell based assay: suitable
flow cytometry: suitable

detection method

fluorometric

shipped in

cooler

storage temp.

−20°C

General description

The BioTracker Membright-640 live cell dye is a “turn-on” fluorescence probe emitting in the far red spectrum for the imaging of plasma membranes of live cells.

Probes specific for the plasma membrane are fundamental tools for marking cell boundaries. Membright probes use a unique mechanism, existing in a self-quenched nanoparticle state. On contact and propagation within the cell membrane, the probe becomes fluorogenic, demonstrating remarkably high signal-to-background ratio. These live cell membrane probes are compatible with two-photon imaging, superresolution imaging, and are suitable for use in live or fixed cells. Their exceptional photostability and lack of cytotoxicity make Membright probes optimal for long-term imaging studies. The key characteristics of these plasma membrane probes permit use in a broad spectrum of fluorescence microscopy applications, and in flow cytometry.

Spectral Properties
Fluorescence images obtained by λex = 640 nm and emission range of 640-800 nm.

Reference

Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614

Application

Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.

Features and Benefits

Far red fluorescent probe for efficient, selective labeling of the plasma membrane in live cells. Suitable for superresolution microscopy.

Physical form

Blue solution

Storage and Stability

Store long term at -20°C, protected from light.

Other Notes

Live cell fluorescent imaging, flow cytometry

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


Certificates of Analysis (COA)

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