SCT152
Firefly/Renilla Dual Luciferase Assay
Flash-type dual luciferase assay that allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity.
Synonym(s):
Firefly Luciferase Assay, Dual Luciferase Assay, Bioluminescent Assay
About This Item
Recommended Products
technique(s)
cell based assay: suitable
detection method
chemiluminescent
shipped in
ambient
General description
The Firefly/Renilla Dual Luciferase Assay allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. Renilla Luciferase Assay Buffer 2.0 quenches the firefly luciferase activity to the level of untransfected cells, allowing sequential measurement of firefly and Renilla luciferase activity in the same sample. This is a flash-type assay that requires luminescence to be measured immediately after adding the detection reagents to the luciferase sample. Firefly signal decays over the course of about 12 minutes, while Renilla signal decays over the course of about 2 minutes, although this may vary depending on enzyme levels.
Application
Cell Signaling
Live Cell Dye
Components
2) Firefly Luciferase Assay Buffer 2.0 (CS224591): 15 mL
3) D-Luciferin (CS224551): 3 X 1 mg
4) Renilla Luciferase Assay Buffer 2.0 (CS224580): 15 mL
5) Aquaphile Coelenterazine (CS224581): 3 X 200 µg
Physical form
Storage and Stability
Disclaimer
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
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Articles
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
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