ST1202
Anti-PGC-1α Mouse mAb (4C1.3)
liquid, clone 4C1.3, Calbiochem®
Synonym(s):
Anti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4C1.3, monoclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
storage temp.
−70°C
target post-translational modification
unmodified
Gene Information
human ... PPARGC1A(10891)
General description
PGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors and regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full-length protein is 113 kDa and is induced in brown adipose tissue by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full-length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals that induce expression of the full-length protein. The N-terminal PGC-1α splice variant is also subject to post translational modifications that alter its migration and apparent molecular weight.
Protein G purified mouse monclonal antibody. Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant.
Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant, in brown adipose tissue, liver, and kidney.
This Anti-PGC-1α Mouse mAb (4C1.3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation, Paraffin Sections for the detection of PGC-1α.
Immunogen
Mouse
a recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
Application
Immunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (10 µg/ml)
Paraffin Sections (whole mount) (see application references)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (10 µg/ml)
Paraffin Sections (whole mount) (see application references)
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Physical form
One vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of NT-PGC-1α Positive Control, supplied as 25 µl whole cell extract in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Endogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.
Lai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Eri Miyamoto-Mikami et al.
Scientific reports, 8(1), 16811-16811 (2018-11-16)
High-intensity intermittent exercise training (HIIT) has been proposed as an effective approach for improving both, the aerobic and anaerobic exercise capacity. However, the detailed molecular response of the skeletal muscle to HIIT remains unknown. We examined the effects of the
Amy E Mendham et al.
Journal of applied physiology (Bethesda, Md. : 1985), 121(6), 1326-1334 (2016-10-16)
This study assessed the mitochondrial related signaling responses to a single bout of noncontact, modified football (touch rugby), played as small-sided games (SSG), or cycling (CYC) exercise in sedentary, obese, middle-aged men. In a randomized, crossover design, nine middle-aged, sedentary
Naoki Horii et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 312(4), R520-R528 (2017-01-27)
The purpose of this study was to investigate the effect of chronic chlorella intake alone or in combination with high-intensity intermittent exercise (HIIE) training on exercise performance and muscle glycolytic and oxidative metabolism in rats. Forty male Sprague-Dawley rats were
Xingyuan Wen et al.
BioMed research international, 2014, 402175-402175 (2014-08-20)
PGC-1α is an inducible transcriptional coactivator that regulates mitochondrial biogenesis and cellular energy metabolism in skeletal muscle. Recent studies have identified two additional PGC-1α transcripts that are derived from an alternative exon 1 (exon 1b) and induced by exercise. Given
Dean-Chuan Wang et al.
International journal of molecular sciences, 21(11) (2020-06-04)
Early-life exposure to di-(2-ethylhexyl)-phthalate (DEHP) has been suggested to relate to hyperactivity, lack of attention, and working memory deficits in school-age children. Brain-derived neurotrophic factor (BDNF) and endocannabinoids are induced by aerobic exercises to provide beneficial effects on brain functions.
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