11699113001
Roche
MgCl2 Stock Solution
pH 8.25-8.45 (25 °C), suitable for PCR, solution, pkg of 3 × 1 mL
Synonym(s):
magnesium chloride solution
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About This Item
UNSPSC Code:
41116100
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form
solution
Quality Level
packaging
pkg of 3 × 1 mL
manufacturer/tradename
Roche
concentration
25 mM
technique(s)
PCR: suitable
pH
8.25-8.45 (25 °C)
storage temp.
−20°C
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General description
The MgCl2 Stock Solution is used for the individual adjustment of the Mg2+-concentration in the PCR reaction and can be used in combination with the PCR buffer without MgCl2. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and the yield of the PCR reaction.
Application
The combination of PCR Buffer without MgCl2 and the MgCl2 Stock Solution buffer are optimized for the amplification of specific DNA fragments in conventional PCRs (i.e., with Taq DNA Polymerase). However, the MgCl2 Stock Solution may be used with other, more appropriate PCR buffers to optimize any type of PCR (e.g., hot start PCR, high fidelity PCR, long template PCR).
Biochem/physiol Actions
Magnesium (Mg2+) chelates nucleotide triphosphates (dNTPs) and its usage is one among the optimization strategies for polymerase chain reaction (PCR). It promotes orthophosphate synthesis from oligophosphates by stabilizing the phosphate groups of nucleotides.
Physical form
The MgCl2 Stock Solution contains 25mM MgCl2 in sterile water.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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flash_point_f
does not flash
flash_point_c
does not flash
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